Chang Cuihua, Ma Yuzhou, Yang Yanjun, Su Yujie, Gu Luping, Li Junhua
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Foods. 2024 Nov 29;13(23):3869. doi: 10.3390/foods13233869.
Collagen peptides play a crucial role in promoting skin elasticity and enhancing joint health, with potential functions to be explored. Enzyme hydrolysis is crucial for the molecular weight and sequence of peptides, influencing the bio-activity. In this study, the angiotensin-converting enzyme (ACE) inhibitory activity and fibroblast proliferation activity of differentially molecular weight peptides derived from dual- or triple-enzyme hydrolysis were compared. Ultrafiltration membrane filtration was used to separate the hydrolyzed prepared collagen peptides into two components based on the molecular size. The results showed that the low-molecular-weight peptide fraction containing peptides with P at the C-terminal, such as KP, RP, and POGP, exhibited high ACE inhibitory activity. The low-molecular-weight peptide fraction obtained through triple-enzyme hydrolysis incorporating ginger protease exhibited the highest ACE inhibitory activity, with an IC50 3.1 mg/mL. In addition, the triple-enzyme hydrolyzed collagen peptides passing across membranes displayed higher migration rates and enhanced collagen synthesis capabilities, containing peptide sequences, such as POGP, POGA, and LPO, potentially promoting fibroblast proliferation. The results would provide practical guidance for the production of collagen peptides with high ACE inhibitory activity and fibroblast proliferation activity, in terms of enzyme processing and highly active peptide separation.
胶原蛋白肽在促进皮肤弹性和增强关节健康方面发挥着关键作用，还有待探索的潜在功能。酶水解对于肽的分子量和序列至关重要，影响着生物活性。在本研究中，比较了双酶或三酶水解得到的不同分子量肽的血管紧张素转换酶（ACE）抑制活性和成纤维细胞增殖活性。采用超滤膜过滤根据分子大小将水解制备的胶原蛋白肽分离为两个组分。结果表明，含有C端带P的肽（如KP、RP和POGP）的低分子量肽组分表现出高ACE抑制活性。通过加入生姜蛋白酶的三酶水解获得的低分子量肽组分表现出最高的ACE抑制活性，IC50为3.1 mg/mL。此外，穿过膜的三酶水解胶原蛋白肽显示出更高的迁移率和增强的胶原蛋白合成能力，含有如POGP、POGA和LPO等肽序列，可能促进成纤维细胞增殖。这些结果将在酶处理和高活性肽分离方面为生产具有高ACE抑制活性和成纤维细胞增殖活性的胶原蛋白肽提供实际指导。
