Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
J Agric Food Chem. 2022 Oct 5;70(39):12663-12671. doi: 10.1021/acs.jafc.2c04659. Epub 2022 Sep 25.
Selenium-enriched egg white peptides (Se-EWP) were prepared by pre-heat treatment and enzymatic hydrolysis in this study. In addition, their selenopeptide sequence identification and immunomodulatory effect were investigated. Results showed that the yield of Se-EWP obtained from alkaline-neutral protease treatment reached 76.90%, and peptides with a molecular weight of 200-1000 Da accounted for 98.33%. Four characteristic selenopeptides, including SeCys-Trp-Leu-Glu, Trp-Ser-SeCys, SeMet-Ala-Pro, and SeMet-Leu, were identified by HPLC-ESI-MS/MS, which were rich in hydrophobic and branched-chain amino acids. Se-EWP (750 mg/kg/d) could effectively retard the decrease of immune organ index in immunosuppressed mice induced by cyclophosphamide. Moreover, supplementation of Se-EWP could promote a higher content of Se in liver, the number of white blood cells, and the levels of serum cytokines (IL-6, IL-2, and TNF-α) as compared with EWP groups, indicating that Se-EWP could effectively alleviate immunosuppression induced by cyclophosphamide. These findings suggested that Se-EWP exhibited great potential as functional foods for immunomodulatory effect.
本研究采用预加热处理和酶解的方法制备富硒蛋清肽（Se-EWP），并对其硒肽序列鉴定和免疫调节作用进行了研究。结果表明，碱性中性蛋白酶处理得到的 Se-EWP 得率为 76.90%，分子量在 200-1000 Da 的肽段占 98.33%。通过 HPLC-ESI-MS/MS 鉴定出 4 种特征硒肽，包括 SeCys-Trp-Leu-Glu、Trp-Ser-SeCys、SeMet-Ala-Pro 和 SeMet-Leu，它们富含疏水性和支链氨基酸。Se-EWP（750mg/kg/d）可有效延缓环磷酰胺诱导的免疫抑制小鼠免疫器官指数的下降。此外，与 EWP 组相比，补充 Se-EWP 可使肝脏中硒的含量、白细胞数量和血清细胞因子（IL-6、IL-2 和 TNF-α）水平更高，表明 Se-EWP 可有效缓解环磷酰胺引起的免疫抑制。这些发现表明，Se-EWP 作为具有免疫调节作用的功能性食品具有很大的潜力。
