Application of Image-Based Phenotyping for QTL Identification of Tiller Angle in Rice ( L.).

Rice tiller angle is a key agronomic trait that regulates plant architecture and plays a critical role in determining rice yield. Given that tiller angle is regulated by multiple genes, it is important to identify quantitative trait loci (QTL) associated with tiller angle. Recently, with the advancement of imaging technology for plant phenotyping, it has become possible to quickly and accurately measure agronomic traits of breeding populations. In this study, we extracted tiller angle and various image-based parameters from Red-Green-Blue (RGB) images of a recombinant inbred line (RIL) population derived from a cross between Milyang23 () and Giho (). Correlations among the obtained data were analyzed, and through dynamic QTL mapping, five major QTLs (, -1, , -1, and ) related to tiller angle were detected on chromosomes 1, 2, and 9. Among them, 26 candidate genes related to auxin signaling and plant growth, including the () gene, were identified in (RM257-STS09048). These results demonstrate the potential of image-based phenotyping to overcome the limitations of traditional manual measurements in crop structure research. Furthermore, the identification of key QTLs and candidate genes related to tiller angle provides valuable genetic insights for the development of high-yielding varieties through crop morphology control.