Application of Microwave-Assisted Water Extraction (MAWE) to Fully Realize Various Physiological Activities of Leaf Extract.

is widely grown in tropical areas worldwide. Studies have demonstrated that extracts of its buds, leaves, and branches obtained through hydrodistillation, steam distillation, or solvent extraction exhibit physiological activities, including anti-melanogenic, antibacterial, and antioxidant properties; nevertheless, such extracts are mostly not effectively collected or adequately utilized. Accordingly, this study applied a rapid, effective, and easy-to-operate microwave-assisted water extraction (MAWE) technique for the first time to prepare leaf extract (MLE) with improved physiological activities. The results indicated that the optimal irradiation time and liquid/solid ratio for the production of the MLE were 180 s and 20 mL/g, respectively. Under optimal conditions, the freeze-dried MLE achieved a high yield (6.28% ± 0.08%) and highly effective broad-spectrum physiological activities. The MLE exhibited strong antioxidant, antiaging, and anti-inflammatory activities and excellent antityrosinase and antimicrobial activities. Additionally, the MLE was noncytotoxic at concentrations of ≤300 mg/L, at which it exhibited pharmacological activity. The results also indicated that the MLE comprised a total of 24 chemical compounds and 17 phenolic compounds. Among these compounds, luteolin contributed to antityrosinase activity. The extract's antiaging activity was attributed to ellagic acid and quercetin, its anti-inflammatory activity resulted from ellagic acid and kaempferol, and its antimicrobial activity resulted from quercetin and 3--methylellagic acid. In conclusion, the MAWE-derived MLE may be useful as a functional ingredient in cosmetic products, health foods, and botanical drugs.