United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Subtropical Horticulture Research Station (SHRS), Miami, FL 33158, USA.
Molecules. 2023 May 6;28(9):3925. doi: 10.3390/molecules28093925.
Tea tree oil (TTO) is a volatile essential oil obtained by distillation, mainly from the Australian native plant (Maiden & Betche) Cheel (Myrtaceae). In this study, a comparative analysis of the chemical constituents of seven tea tree oils () and four other spp. oils (, (M), two chemotypes of , (M and M), and (M)) was carried out using gas chromatography-mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC). Among the seven TTOs, terpinen-4-ol (37.66-44.28%), γ-terpinene (16.42-20.75%), α-terpinene (3.47-12.62%), α-terpineol (3.11-4.66%), and terpinolene (2.75-4.19%) were the most abundant compounds. On the other hand, the most abundant compounds of the other oils varied, such as 1,8-cineole (64.63%) in M oil, (E)-nerolidol (48.40%) and linalool (33.30%) in M oil, 1,8-cineole (52.20%) in M oil, and linalool (38.19%) and 1,8-cineole (27.57%) in M oil. HPTLC fingerprinting of oils enabled the discrimination of TTO oils from other spp. oils. Variation was observed in the profile of the R values among EOs. The present study shows that HPTLC is one of the best ways to identify and evaluate the quality control in authenticating TTOs, other EOs, or EOs from other species within the Myrtaceae.
茶树油（TTO）是一种挥发性精油，通过蒸馏获得，主要来自澳大利亚本地植物（Maiden & Betche）Cheel（桃金娘科）。在这项研究中，使用气相色谱-质谱联用（GC-MS）和高效薄层色谱（HPTLC）对七种茶树油（）和其他四种 spp.油（，（M），两种化学型的 ，（M 和 M），和 （M））的化学成分进行了比较分析。在这七种 TTO 中，萜品-4-醇（37.66-44.28%）、γ-萜品烯（16.42-20.75%）、α-萜品烯（3.47-12.62%）、α-萜品醇（3.11-4.66%）和萜品烯（2.75-4.19%）是最丰富的化合物。另一方面，其他 油中最丰富的化合物各不相同，例如 M 油中的 1,8-桉叶素（64.63%），M 油中的（E）-香叶醇（48.40%）和芳樟醇（33.30%），M 油中的 1,8-桉叶素（52.20%）和 M 油中的芳樟醇（38.19%）和 1,8-桉叶素（27.57%）。 油的 HPTLC 指纹图谱可区分 TTO 油与其他 spp.油。在 EO 之间观察到 R 值谱的变化。本研究表明，HPTLC 是识别和评估 TTO 油、其他 油或桃金娘科其他物种 EO 质量控制的最佳方法之一。
