Xie Lihua, Li Xiangyu, Yao Pengqiang, Cheng Zhanchao, Cai Miaomiao, Liu Chunyang, Wang Zhe, Gao Jian
Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China.
Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, China.
Plants (Basel). 2024 Dec 9;13(23):3452. doi: 10.3390/plants13233452.
NAC (NAM, ATAF, and CUC) transcription factors are essential in regulating plant stress response and senescence, with their functions being modulated by alternative splicing. The molecular mechanisms of stress-induced premature flowering and drought tolerance in (moso bamboo) are not yet fully understood. In this study, a novel NAC variant derived from , named , was isolated. PheNAC23 exhibited distinct expression patterns compared to PheNAC23 during leaf senescence and drought stress response. Overexpression of promoted flowering and reduced its tolerance to drought stress in (). However, overexpression of exhibited the opposite functions. PheNAC23 was localized in the nucleus and had transactivation activity, while PheNAC23 had a similar localization to the control green fluorescent protein and no transactivation activity. Further functional analysis revealed that PheNAC23 could interact with PheNAC23, suggesting that PheNAC23 might serve as a small interfering peptide that affects the function of PheNAC23 by binding to it.
NAC（NAM、ATAF和CUC）转录因子在调节植物应激反应和衰老过程中至关重要，其功能受可变剪接调控。毛竹中应激诱导的早花和耐旱性的分子机制尚未完全明确。在本研究中，从毛竹中分离出一种源自PheNAC23的新型NAC变体，命名为PheNAC23。在叶片衰老和干旱胁迫响应过程中，PheNAC23与PheNAC23表现出不同的表达模式。过表达PheNAC23促进了毛竹开花并降低了其对干旱胁迫的耐受性。然而，过表达PheNAC23则表现出相反的功能。PheNAC23定位于细胞核且具有反式激活活性，而PheNAC23与对照绿色荧光蛋白具有相似的定位且无反式激活活性。进一步的功能分析表明，PheNAC23可与PheNAC23相互作用，这表明PheNAC23可能作为一种小干扰肽，通过与PheNAC23结合来影响其功能。
