Tognacca Rocío S, Rodríguez Florencia S, Aballay Federico E, Cartagena Carla M, Servi Lucas, Petrillo Ezequiel
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina.
J Exp Bot. 2023 Apr 9;74(7):2251-2272. doi: 10.1093/jxb/erac431.
Alternative splicing is an important regulatory process that produces multiple transcripts from a single gene, significantly modulating the transcriptome and potentially the proteome, during development and in response to environmental cues. In the first part of this review, we summarize recent advances and highlight the accumulated knowledge on the biological roles of alternative splicing isoforms that are key for different plant responses and during development. Remarkably, we found that many of the studies in this area use similar methodological approaches that need to be improved to gain more accurate conclusions, since they generally presume that stable isoforms undoubtedly have coding capacities. This is mostly done without data indicating that a particular RNA isoform is in fact translated. So, in the latter part of the review, we propose a thorough strategy to analyze, evaluate, and characterize putative functions for alternative splicing isoforms of interest.
可变剪接是一种重要的调控过程，它从单个基因产生多个转录本，在发育过程中以及对环境信号的响应中显著调节转录组，并可能调节蛋白质组。在本综述的第一部分，我们总结了最近的进展，并强调了关于可变剪接异构体生物学作用的积累知识，这些异构体对于不同的植物反应和发育过程至关重要。值得注意的是，我们发现该领域的许多研究使用了类似的方法学方法，这些方法需要改进以获得更准确的结论，因为它们通常假定稳定的异构体无疑具有编码能力。这样做大多没有数据表明特定的RNA异构体实际上被翻译了。因此，在综述的后半部分，我们提出了一种全面的策略，用于分析、评估和表征感兴趣的可变剪接异构体的推定功能。
