通过荧光和双光子显微镜获得的刺果菊属果实中的链烷酰胺含量及定位
Alkamide Content and Localization in Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy.
作者信息
Vázquez-Martínez Juan, Molina-Torres Jorge
机构信息
Departamento de Ingeniería Química, TecNM/ITS Irapuato, Silao-Irapuato Km 12.5 El Copal, Irapuato CP 36821, GTO, Mexico.
Departamento de Biotecnología y Bioquímica, Cinvestav Irapuato, Km. 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato CP 36821, GTO, Mexico.
出版信息
Molecules. 2024 Nov 29;29(23):5651. doi: 10.3390/molecules29235651.
The alkamide content and specific tissue localization in the cypselae of were investigated using gas chromatography-electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and -2-methylbutyl-2,6,8-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization. The linear unmixing of fluorescence emission fingerprints showed that affinin exhibits autofluorescence at 693 nm, corresponding to the red spectral region. This emission is attributed to the conjugated double bonds in its acyl chain. This study is the first to report on the presence and precise localization of alkamides in the cypselae of
使用气相色谱 - 电子电离质谱（GC - EIMS）和多光子荧光显微镜（MPFM）研究了[植物名称]瘦果中的烷酰胺含量和特定组织定位。GC - EIMS分析鉴定出两种烯属烷酰胺：阿非宁（毛果芸香碱）和 - 2 - 甲基丁基 - 2,6,8 - 癸三烯酰胺。显微镜分析表明，烷酰胺定位于子叶内，并特别分隔在脂质体中，突出了它们的空间组织。荧光发射指纹图谱的线性解混显示，阿非宁在693nm处呈现自发荧光，对应于红色光谱区域。这种发射归因于其酰基链中的共轭双键。本研究首次报道了[植物名称]瘦果中烷酰胺的存在及其精确定位。
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