Xu Hua, Huang Mengfan, Zhou Mengyuan, Guo Rong, Qin Kunming, Dong Zibo
School of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China.
Southern Medical University, Guangzhou 511453, China.
Molecules. 2024 Nov 30;29(23):5681. doi: 10.3390/molecules29235681.
Silk microfiber scaffolds have garnered increasing interest due to their outstanding properties, with degumming being the process used to extract the sericin from the cocoon. In the present study, an attempt to tune the biodegradation period of silk through degumming with various sodium borohydride (NaBH) concentrations and degumming times was studied. We considered the process, the number of baths used, and the salt concentration. Herein, we report a novel method of expanding microfibers from two-dimensional (2D) to three-dimensional (3D) using a modified gas-foaming technique. Porous three-dimensional (3D) silk fibroin (SF) scaffolds were fabricated by the SF fibers, which were extracted by the NaBH degumming method and NaBH gas-foaming approach. This study showed that higher salt concentrations, reaching 1.5% in a double bath, effectively removed sericin from silk fibroin, resulting in clean, smooth 3D scaffolds. These scaffolds were then fabricated using a freeze-drying method. The scaffolds were then submerged in solutions containing semen cuscutae (SC) and their surfaces were coated with various percentages of total flavonoids. The scaffolds had no toxicity to the cells in vitro. This work provides a new route for achieving a TFSC-loaded scaffold; it is proved that the coated silk fibroin fiber scaffold has excellent compatibility. Compared with non-drug-loaded silk scaffolds, drug-loaded silk scaffolds promote cell growth.
丝微纤维支架因其出色的性能而越来越受到关注，脱胶是从蚕茧中提取丝胶蛋白的过程。在本研究中，尝试通过用不同浓度的硼氢化钠（NaBH）脱胶和不同的脱胶时间来调节丝的生物降解期。我们考虑了脱胶过程、脱胶浴的次数和盐浓度。在此，我们报告了一种使用改进的气体发泡技术将微纤维从二维（2D）扩展到三维（3D）的新方法。通过硼氢化钠脱胶法和硼氢化钠气体发泡法提取的丝素纤维制备了多孔三维（3D）丝素蛋白（SF）支架。本研究表明，在双浴中盐浓度达到1.5%时，能有效地从丝素蛋白中去除丝胶蛋白，从而得到干净、光滑的三维支架。然后使用冷冻干燥法制备这些支架。接着将支架浸入含有菟丝子（SC）的溶液中，并在其表面涂覆不同百分比的总黄酮。这些支架在体外对细胞没有毒性。这项工作为制备负载总黄酮丝素纤维支架提供了一条新途径；证明了涂覆丝素纤维支架具有优异的相容性。与未载药的丝支架相比，载药丝支架促进细胞生长。
