Ge Caicai, Chen Fangping, Mao Lijie, Liang Qing, Su Yan, Liu Changsheng
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology Shanghai 200237 P. R. China
Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology Shanghai 200237 P. R. China
RSC Adv. 2020 Mar 2;10(15):9016-9025. doi: 10.1039/c9ra08909h. eCollection 2020 Feb 27.
It is of considerable significance to fabricate scaffolds with satisfactory osteogenic activities and high osteogenesis quality to accelerate osteoporotic repair. In this study, we initially fabricated the POFC/β-TCP porous scaffold in the light of composition and structure bionics, and then loaded the SR to the optimized POFC/β-TCP porous scaffold by 3D printing based on FFS-MDJ. The hydrophilicity, mechanical properties biodegradability and cell response of the composite scaffolds were systematically investigated. The result showed that modified POFC enhanced the hydrophilicity and ameliorated the brittleness of pure β-TCP. β-TCP buffered the acidity and improved the degradability and cell affinity of the scaffold, and the release of strontium ranelate significantly promote the proliferation and differentiation of osteoblasts and guided bone regeneration. The results indicated that POFC/β-TCP scaffolds had uniform macropores of 300-500 μm and a porosity of approximately 48%, adjustable biodegradability and a high compressive modulus of 30-60 MPa. The strontium ranelate-loaded POFC/β-TCP scaffold enhanced the osteogenic differentiation of rBMSCs, which might be a promising candidate for osteoporotic-related bone defect repair.
制备具有良好成骨活性和高成骨质量的支架对于加速骨质疏松修复具有重要意义。在本研究中，我们首先根据成分和结构仿生原理制备了POFC/β-TCP多孔支架，然后基于FFS-MDJ通过3D打印将锶盐（SR）负载到优化后的POFC/β-TCP多孔支架上。系统研究了复合支架的亲水性、力学性能、生物降解性和细胞反应。结果表明，改性后的POFC提高了亲水性，改善了纯β-TCP的脆性。β-TCP缓冲了酸度，提高了支架的降解性和细胞亲和力，雷奈酸锶的释放显著促进了成骨细胞的增殖和分化，并引导骨再生。结果表明，POFC/β-TCP支架具有300-500μm的均匀大孔，孔隙率约为48%，可调节的生物降解性和30-60MPa的高压缩模量。负载雷奈酸锶的POFC/β-TCP支架增强了大鼠骨髓间充质干细胞（rBMSCs）的成骨分化，这可能是骨质疏松相关骨缺损修复的一个有前途的候选材料。
