Ivan Alexandra, Lukinich-Gruia Alexandra Teodora, Cristea Iustina-Mirabela, Pricop Maria-Alexandra, Calma Crenguta Livia, Simina Alina-Georgiana, Tatu Călin Adrian, Galuscan Atena, Păunescu Virgil
Department of Functional Sciences, Center of Immuno-Physiology (CIFBIOTEH), University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania.
OncoGen Centre, Clinical County Hospital "Pius Branzeu", Blvd. Liviu Rebreanu 156, 300723 Timisoara, Romania.
Molecules. 2024 Dec 5;29(23):5755. doi: 10.3390/molecules29235755.
Quercetin is a natural flavonoid renowned for its potent antioxidant, anti-inflammatory, anti-diabetic, and antibacterial properties, making it a highly promising candidate for the treatment of various medical conditions. Our current study investigates the influence of quercetin on energy metabolism, fatty acid composition, oxidative stress gene expression, and sirtuin expression in early- and late-stage passages of stem cells derived from human exfoliated deciduous teeth (SHEDs). Mitochondrial respiration was analyzed by measuring oxygen consumption following a 24 h quercetin treatment, while fatty acid profiles were examined using gas chromatography-mass spectrometry (GC-MS). Additionally, quantitative PCR (qPCR) was used to assess the expression of oxidative stress genes and sirtuins. In younger SHEDs, quercetin enhances metabolic activity and mitochondrial respiration, although higher doses may decrease mitochondrial activity. Conversely, in older, senescent SHEDs, quercetin supports mitochondrial function at lower concentrations but appears to inhibit respiration at higher doses. These results suggest that quercetin may hold therapeutic potential for maintaining SHED viability and function, especially at lower doses in older cells. Further research is essential to fully elucidate a dose-dependent effect of quercetin and optimize its applications in regenerative medicine.
槲皮素是一种天然黄酮类化合物，以其强大的抗氧化、抗炎、抗糖尿病和抗菌特性而闻名，使其成为治疗各种医疗疾病的极具潜力的候选药物。我们目前的研究调查了槲皮素对源自人脱落乳牙（SHEDs）的干细胞早期和晚期传代过程中能量代谢、脂肪酸组成、氧化应激基因表达和沉默调节蛋白表达的影响。在槲皮素处理24小时后，通过测量氧气消耗来分析线粒体呼吸，同时使用气相色谱 - 质谱联用仪（GC-MS）检测脂肪酸谱。此外，定量聚合酶链反应（qPCR）用于评估氧化应激基因和沉默调节蛋白的表达。在较年轻的SHEDs中，槲皮素可增强代谢活性和线粒体呼吸，尽管较高剂量可能会降低线粒体活性。相反，在较老的、衰老的SHEDs中，槲皮素在较低浓度下支持线粒体功能，但在较高剂量下似乎会抑制呼吸。这些结果表明，槲皮素可能具有维持SHED活力和功能的治疗潜力，尤其是在老年细胞中使用较低剂量时。进一步的研究对于充分阐明槲皮素的剂量依赖性效应并优化其在再生医学中的应用至关重要。
