Yu Xiaolei, Wu Haowei, Zhang Lei, Fei Dongliang
Meat Processing and Safety Control Professional Technology Innovation Center, Jinzhou Medical University, Jinzhou 121000, China.
MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Molecules. 2024 Dec 6;29(23):5778. doi: 10.3390/molecules29235778.
Response surface methodology (RSM) was used to optimize the extraction process of naringin. The central component design included three parameters of extraction, namely temperature (X), solid-liquid ratio (X), and extraction time (X). The optimum extraction temperature was 67 °C; the ratio of feed to solvent was 54:1 mL/g, and the extraction time was 2.8 h. According to the best extraction conditions, naringin was processed to verify the accuracy of the model. Five parallel experiments were set up, and a yield of 3.248% naringin was obtained, which was equivalent to the predicted yield of 3.256%. Naringin was purified to obtain naringin-refined products using DM101 macroporous adsorption resin. Naringin dihydrochalcone was synthesized following catalytic hydrogenation of purified naringin. The structures of naringin and naringin dihydrochalcone were determined via Fourier infrared spectrometer and nuclear magnetic resonance spectrometry. In vitro determination of the lipid-lowering activity of naringin dihydrochalcone was also conducted. Further focusing on HepG2 cells, a high cholesterol-induced high-fat HepG2 cell model was established. We measured the effects of different concentrations of naringin dihydrochalcone on intracellular lipids in denatured HepG2 cells and further validated the lipid-lowering effect of naringin at the cellular level. The results showed that naringin dihydrochalcone has a potential application in functional foods for lowering blood lipids.
采用响应面法（RSM）优化柚皮苷的提取工艺。中心组合设计包括提取的三个参数，即温度（X₁）、固液比（X₂）和提取时间（X₃）。最佳提取温度为67℃；料液比为54:1 mL/g，提取时间为2.8 h。根据最佳提取条件，对柚皮苷进行处理以验证模型的准确性。设置了5次平行实验，获得了3.248%的柚皮苷得率，与预测得率3.256%相当。使用DM101大孔吸附树脂对柚皮苷进行纯化，得到柚皮苷精制产品。对纯化后的柚皮苷进行催化氢化反应，合成柚皮苷二氢查耳酮。通过傅里叶红外光谱仪和核磁共振光谱仪测定柚皮苷和柚皮苷二氢查耳酮的结构。还进行了柚皮苷二氢查耳酮体外降脂活性的测定。进一步聚焦于HepG2细胞，建立了高胆固醇诱导的高脂HepG2细胞模型。我们测定了不同浓度的柚皮苷二氢查耳酮对变性HepG2细胞内脂质的影响，并在细胞水平上进一步验证了柚皮苷的降脂效果。结果表明，柚皮苷二氢查耳酮在降血脂功能食品中具有潜在的应用价值。
