MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Meat Processing and Safety Control Professional Technology Innovation Center, Jinzhou Medical University, Jinzhou 121000, China.
Molecules. 2023 Feb 14;28(4):1788. doi: 10.3390/molecules28041788.
The naringin extraction process was optimised using response surface methodology (RSM). A central component design was adopted, which included four parameters: extraction temperature (X), material-liquid ratio (X), extraction time (X), and ultrasonic frequency (X) of 74.79 °C, 1.58 h, 1:56.51 g/mL, and 28.05 KHz, respectively. Based on these optimal extraction conditions, naringin was tested to verify the model's accuracy. Naringin yield was 36.2502 mg/g, which was equivalent to the predicted yield of 36.0124 mg/g. DM101 macroporous adsorption resin was used to purify naringin. The effects of loading concentration, loading flow rate, and sample pH on the adsorption rate of naringin and the effect of ethanol concentration on the desorption rate of naringin were investigated. The optimum conditions for naringin purification using macroporous resins were determined. The optimal loading concentration, sample solution pH, and loading flow rate were 0.075 mg/mL, 3.5, and 1.5 mL/min, respectively. Three parallel tests were conducted under these conditions, and the average naringin yield was 77.5643%. Naringin's structure was identified using infrared spectroscopy and nuclear magnetic resonance. In vitro determination of the lipid-lowering activity of naringin was also conducted. These results showed that naringin has potential applications as a functional food for lowering blood lipid levels.
采用响应面法（RSM）对柚皮苷提取工艺进行优化。采用中心组合设计，包含四个参数：提取温度（X）、料液比（X）、提取时间（X）和超声频率（X），分别为 74.79°C、1.58 h、1:56.51 g/mL 和 28.05 KHz。基于这些最佳提取条件，对柚皮苷进行测试以验证模型的准确性。柚皮苷的产率为 36.2502 mg/g，与预测产率 36.0124 mg/g 相当。采用 DM101 大孔吸附树脂对柚皮苷进行纯化。考察了上样浓度、上样流速和样品 pH 值对柚皮苷吸附率的影响，以及洗脱剂乙醇浓度对柚皮苷解吸率的影响。确定了大孔树脂纯化柚皮苷的最佳条件。最佳上样浓度、样品溶液 pH 值和上样流速分别为 0.075 mg/mL、3.5 和 1.5 mL/min。在这些条件下进行了 3 次平行试验，柚皮苷的平均收率为 77.5643%。采用红外光谱和核磁共振对柚皮苷的结构进行了鉴定。还进行了柚皮苷体外降血脂活性的测定。结果表明，柚皮苷作为一种具有降血脂功能的功能性食品具有潜在的应用前景。
