Stanciu Magdalena-Cristina, Ionita Daniela, Tȋmpu Daniel, Popescu Irina, Suflet Dana Mihaela, Doroftei Florica, Tuchilus Cristina G
"Petru Poni" Institute of Macromolecular Chemistry, 41A, Gr. Ghica-Voda Alley, 700487 Iasi, Romania.
Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 16, University Street, 700115 Iasi, Romania.
Polymers (Basel). 2024 Nov 29;16(23):3352. doi: 10.3390/polym16233352.
New quaternary ammonium derivatives (quats) based on apple pectin (PA) were synthesized by the chemical modification of native polysaccharides with various quaternization mixtures containing epichlorohydrin (ECH) and a tertiary amine. Pectin derivatives (QPAs) were studied by elemental analysis, conductometric titration, Fourier-transform infrared spectroscopy (FTIR), and C nuclear magnetic resonance (C NMR). Viscosity measurements enabled the evaluation of the viscosity average molar mass (M) for the unmodified polysaccharide, as well as its intrinsic viscosity ([η]) value. Dynamic light scattering (DLS) analysis revealed that the PA and its quats formed aggregates in an aqueous solution with either a unimodal (PA) or bimodal (QPAs) distribution. Scanning transmission electron microscopy analysis (STEM) of the PA and its derivatives demonstrated the presence of individual polymeric chains and aggregates in aqueous solution, with the smallest sizes being specific to amphiphilic polymers. Thermal stability, as well as wide-angle X-ray diffraction (WAXD) studies, generally indicated a lower thermal stability and crystallinity of the QPAs compared with those of the PA. Antipathogenic activity demonstrated that the PA and its derivatives exhibited effectiveness against ATCC 25923 bacterium and ATCC 10231 pathogenic yeast.
通过用含有环氧氯丙烷（ECH）和叔胺的各种季铵化混合物对天然多糖进行化学改性，合成了基于苹果果胶（PA）的新型季铵衍生物（季铵盐）。通过元素分析、电导滴定、傅里叶变换红外光谱（FTIR）和碳核磁共振（C NMR）对果胶衍生物（QPA）进行了研究。粘度测量能够评估未改性多糖的粘均摩尔质量（M）及其特性粘度（[η]）值。动态光散射（DLS）分析表明，PA及其季铵盐在水溶液中形成聚集体，具有单峰（PA）或双峰（QPA）分布。对PA及其衍生物的扫描透射电子显微镜分析（STEM）表明，水溶液中存在单个聚合物链和聚集体，最小尺寸特定于两亲性聚合物。热稳定性以及广角X射线衍射（WAXD）研究通常表明，与PA相比，QPA的热稳定性和结晶度较低。抗病原活性表明，PA及其衍生物对ATCC 25923细菌和ATCC 10231致病酵母具有有效性。
