Ding Fuyuan, Shi Xiaowen, Li Xiaoxia, Cai Jie, Duan Bo, Du Yumin
Department of Environmental Science, College of Resource and Environmental Science, Wuhan University, Wuhan 430072, China.
Department of Environmental Science, College of Resource and Environmental Science, Wuhan University, Wuhan 430072, China.
Carbohydr Polym. 2012 Jan 4;87(1):422-426. doi: 10.1016/j.carbpol.2011.07.069. Epub 2011 Aug 10.
Water-soluble and white quaternized chitin (QC) was homogeneously synthesized by stirring transparent chitin solution (2%) in 8wt%NaOH/4wt% urea aqueous solution containing 2,3-Epoxypropyltrimethylammonium Chloride (EPTMAC) at 10°C for 24h. The structure and properties of quaternized chitin were characterized by FT-IR, XRD, H NMR, GPC, element analysis and ζ-potential. The results indicate that quaternary groups were successfully incorporated onto chitin backbones and the degree of substitution (DS) of quaternary groups can be easily adjusted by changing the molar ratio of chitin unit to EPTMAC. Additionally, quaternized chitin shows better antibacterial activity against Escherichia coli and Staphylococcus aureus as compared with chitosan. Thus, this work provides a simply and "green" method to functionalize chitin and the resulting quaternized chitin may have potential applications in environmental, food and biomedical fields.
通过在含有2,3-环氧丙基三甲基氯化铵（EPTMAC）的8wt%氢氧化钠/4wt%尿素水溶液中，于10°C搅拌2%的透明甲壳素溶液24小时，均匀合成了水溶性白色季铵化甲壳素（QC）。采用傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、核磁共振氢谱（H NMR）、凝胶渗透色谱（GPC）、元素分析和ζ电位对季铵化甲壳素的结构和性能进行了表征。结果表明，季铵基团成功地引入到甲壳素主链上，通过改变甲壳素单元与EPTMAC的摩尔比，可以轻松调节季铵基团的取代度（DS）。此外，与壳聚糖相比，季铵化甲壳素对大肠杆菌和金黄色葡萄球菌表现出更好的抗菌活性。因此，这项工作提供了一种简单且“绿色”的方法来对甲壳素进行功能化，所得的季铵化甲壳素可能在环境、食品和生物医学领域具有潜在应用。
