Bergqvist A, Jeppsson S, Ljungberg O
J Histochem Cytochem. 1985 Feb;33(2):155-61. doi: 10.1177/33.2.3968421.
Estrogen and progesterone binding to endometriotic and endometrial tissue was studied histochemically using estradiol and progesterone fluorochrome derivatives (E2-bovine serum albumin-fluorescein isothiocyanate and progesterone-bovine serum albumin-tetramethylrhodamine isothiocyanate). Thirty endometriotic samples from 21 women were studied, together with endometrial specimens obtained simultaneously from 14 of the women. In 77% of the endometriotic samples binding of the estrogen conjugate was indicated by specific fluorescence in more than half of the epithelial cell population, and in 20% in less than half. The corresponding figures for the progesterone conjugate binding were 75 and 18%, respectively. Blocking studies indicated a reasonable degree of ligand specificity. In endometrial tissue the corresponding figures were 64 and 29%, respectively, for binding of the estrogen conjugate and 54 and 38%, respectively, for binding of the progesterone conjugate. In 7 of 13 cases where evaluable samples of both tissues had been obtained, the relative proportion of fluorescent cells, with either reagent, was similar in the two tissue types. Our results suggest that the cytoplasm of epithelial cells in endometriotic tissue and in uterine endometrium contains specific binding sites for both estrogen and progesterone. The binding pattern of the two conjugates in endometriotic tissue was unrelated to the menstrual phase.
使用雌二醇和孕酮荧光染料衍生物（雌二醇 - 牛血清白蛋白 - 异硫氰酸荧光素和孕酮 - 牛血清白蛋白 - 异硫氰酸四甲基罗丹明）对子宫内膜异位症组织和子宫内膜组织中的雌激素和孕酮结合进行了组织化学研究。研究了来自21名女性的30个子宫内膜异位症样本，以及同时从其中14名女性获得的子宫内膜标本。在77%的子宫内膜异位症样本中，超过一半的上皮细胞群体出现特异性荧光，表明雌激素结合物与之结合，20%的样本中少于一半的上皮细胞群体出现特异性荧光。孕酮结合物结合的相应数字分别为75%和18%。阻断研究表明配体具有合理程度的特异性。在子宫内膜组织中，雌激素结合物结合的相应数字分别为64%和29%，孕酮结合物结合的相应数字分别为54%和38%。在13例同时获得两种组织可评估样本的病例中，有7例两种组织类型中使用任何一种试剂时荧光细胞的相对比例相似。我们的结果表明，子宫内膜异位症组织和子宫内膜上皮细胞的细胞质中都含有雌激素和孕酮的特异性结合位点。子宫内膜异位症组织中两种结合物的结合模式与月经周期无关。
