Zhang Li, Jing Panpan, Geng Biao, Zhang Jinlong, Shi Jinjiang, Liang Dong, Yang Yujie, Qu Yunfang, Huang Jinling
College of Agriculture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Int J Mol Sci. 2024 Nov 26;25(23):12707. doi: 10.3390/ijms252312707.
Abnormal programmed cell death in the tapetum is induced by reactive oxygen species (ROS), which are the main factors leading to cytoplasmic male sterility (CMS). These abnormalities are caused by genetic interactions between nuclear and cytoplasmic genes. To explore the role of chloroplast genes in ROS metabolism, next-generation and single-molecule real-time sequencing of the chloroplast genome were performed in the cotton CMS line Jin A (Jin A-CMS). Our results showed that the chloroplast genome is 160,042 bp in length and consists of 131 genes, including 112 functional genes. An analysis of the functional annotation and sequence comparison with the chloroplast genome as a reference revealed that 29 genes in Jin A-CMS have single-nucleotide polymorphisms, including subunits of ATP synthase, NAD(P)H-quinone redox reductase, and photosystem complexes. Compared to the Jin B maintainer, the anthers of Jin A-CMS at the microspore abortion stage have significantly lower expression of , , and . The relative expression of these genes is significantly higher in the three-line F1 hybrids compared to Jin A-CMS. The ROS levels in the leaves increased in response to the silencing of and in cotton plants. In summary, the results of our study show that the ATP synthase subunit genes and are closely linked with ROS metabolism. These results provide basic information for the functional analysis of ATP synthase in cotton.
绒毡层中异常的程序性细胞死亡是由活性氧（ROS）诱导的，活性氧是导致细胞质雄性不育（CMS）的主要因素。这些异常是由核基因和细胞质基因之间的遗传相互作用引起的。为了探究叶绿体基因在ROS代谢中的作用，对棉花CMS系晋A（晋A-CMS）进行了叶绿体基因组的二代测序和单分子实时测序。我们的结果表明，叶绿体基因组长度为160,042 bp，由131个基因组成，其中包括112个功能基因。以叶绿体基因组为参考进行功能注释和序列比较分析发现，晋A-CMS中有29个基因存在单核苷酸多态性，包括ATP合酶、NAD(P)H-醌氧化还原酶和光系统复合物的亚基。与晋B保持系相比，晋A-CMS在小孢子败育阶段的花药中，[此处原文缺失基因名称]、[此处原文缺失基因名称]和[此处原文缺失基因名称]的表达显著降低。与晋A-CMS相比，这些基因在三系F1杂种中的相对表达显著更高。棉花植株中[此处原文缺失基因名称]和[此处原文缺失基因名称]沉默后，叶片中的ROS水平升高。总之，我们的研究结果表明，ATP合酶亚基基因[此处原文缺失基因名称]和[此处原文缺失基因名称]与ROS代谢密切相关。这些结果为棉花中ATP合酶的功能分析提供了基础信息。
