Is Fluoride Blameless?-The Influence of Fluorine Compounds on the Invasiveness of the Human Glioma-like Cell Line U-87.

Glioblastoma remains one of the most treatment-resistant and malignant human cancers. Given the documented harmful effects of fluoride on the developing central nervous system and the rising incidence of brain tumors, especially among children, it is pertinent to explore the role of environmental toxins, including fluoride compounds, in the context of brain cancer. This study represents the first investigation into the influence of fluoride on mechanisms related to the invasiveness of human glioblastoma cells. We examined the effects of sodium fluoride (NaF) exposure on the migratory and invasive abilities of the U-87 human glioblastoma cell line, assessing levels of metalloproteinases MMP-2 and MMP-9 secreted by these cells. Additionally, the activation of metabolic pathways associated with invasiveness, including AKT and NF-κB, was analyzed. Our results suggest that the effects induced by NaF at physiologically high concentrations (0.1-10 µM) in U-87 glioblastoma cells may promote a pro-invasive phenotype.