Li Yunjiao, Li Zecheng, Ran Hongmei, Fan Zihan, Yang Fan, Chen Hu, Zhou Bo
Fisheries Research Institute of Sichuan Academy of Agricultural Sciences, Yibin 644000, China.
Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, School of Marine Biology and Fisheries, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Haikou 570228, China.
Int J Mol Sci. 2024 Nov 28;25(23):12813. doi: 10.3390/ijms252312813.
, with an annual production of 5-6 million tons and a value of USD 50-60 billion, is a cornerstone of global aquaculture. However, molting-related losses of 5-20% significantly impact this industry, and the physiological mechanisms of molting remain unclear. This study aims to elucidate the role of eclosion hormone (EH) in molting regulation and enhances the understanding of molting physiology in . This study investigated the role of (EH) in molting regulation. Two cDNAs, and , were identified, and their expression patterns across tissues and seven molting stages (A, B, C, D0, D1, D2, and D3) were analyzed. was predominantly expressed in the gill, epidermis, and eyestalk, while was mainly expressed in the eyestalk and brain. was highly expressed in the eyestalk, epidermis, and gills at the D2 and D3 stages of molting, whereas was highly expressed in both the D2 (brain) and D3 (eyestalk) stages. RNA interference (RNAi) targeting revealed its critical role in molting, as silencing disrupted the expression of molting-regulation genes, , , , , , and bursicon (), significantly delaying the molting process. These findings highlight both and as indispensable for normal molting in and provide a foundation for developing effective molting management strategies to reduce industry losses.
年产量为500 - 600万吨，价值500 - 600亿美元，是全球水产养殖的基石。然而，与蜕皮相关的5% - 20%的损失对该行业产生了重大影响，且蜕皮的生理机制仍不清楚。本研究旨在阐明羽化激素（EH）在蜕皮调节中的作用，并增进对[物种名称未明确]蜕皮生理学的理解。本研究调查了[物种名称未明确]中羽化激素（EH）在蜕皮调节中的作用。鉴定出两个[物种名称未明确]的cDNA，[具体名称1]和[具体名称2]，并分析了它们在不同组织以及七个蜕皮阶段（A、B、C、D0、D1、D2和D3）的表达模式。[具体名称1]主要在鳃、表皮和眼柄中表达，而[具体名称2]主要在眼柄和脑中表达。[具体名称1]在蜕皮的D2和D3阶段在眼柄、表皮和鳃中高表达，而[具体名称2]在D2阶段（脑）和D3阶段（眼柄）均高表达。针对[具体名称1]的RNA干扰（RNAi）揭示了其在蜕皮中的关键作用，因为沉默[具体名称1]会破坏蜕皮调节基因[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]、[具体基因5]、[具体基因6]和羽化肽（[具体名称3]）的表达，显著延迟蜕皮过程。这些发现突出了[具体名称1]和[具体名称2]对于[物种名称未明确]正常蜕皮的不可或缺性，并为制定有效的蜕皮管理策略以减少行业损失提供了基础。
