Key Neuropeptides Regulating Molting in Pacific White Shrimp (): Insights from Transcriptomic Analysis.

Molting is a vital physiological process essential for the growth and development of , with significant implications for aquaculture productivity. This study aimed to identify neuropeptide-related genes involved in molting through transcriptomic analysis. RNA sequencing of pre-molt and post-molt samples revealed 1203 differentially expressed genes (DEGs). Functional enrichment analysis indicated that these genes play significant roles in cuticle formation and molting regulation. Among the DEGs, 243 were predicted to be neuropeptides based on the presence of signal peptides and the absence of transmembrane domains. Five key neuropeptide genes-, , I, I, and II-were identified as critical regulators of molting. Their role was further validated through RT-qPCR analysis, confirming their close association with the molting process. These genes were highlighted in this study as pivotal factors driving molting in The neuropeptides identified in this research are anticipated to offer valuable insights into the regulation of molting. Additionally, their synthetic products hold promise for improving molting consistency in shrimp aquaculture.