Li Ling, Yao Zhihan, Pang Baoping, Li Yanyan
Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China.
Int J Mol Sci. 2024 Dec 3;25(23):12976. doi: 10.3390/ijms252312976.
20-hydroxyecdysone (20E) signaling plays an important role in regulating insect growth, development, and reproduction. However, the effect of 20E on reproductive diapause and its regulatory mechanisms have not been fully understood. is a new pest in the Inner Mongolia grasslands, and it aestivates in an obligatory reproductive diapause form. In this study, the complete open reading frame (ORF) sequence of the () was cloned from . Application of 20E promoted the expression of , (), and (), whereas it reduced the expression of () and lipid content, leading to delayed diapause entry in female adults. Silencing or by RNAi increased the expression of and lipid content, whereas it reduced the expression of , inducing reproductive diapause. These results indicate that 20E may mediate reproductive diapause via a conserved EcR/HR3 cascade in .
20-羟基蜕皮酮（20E）信号传导在调节昆虫生长、发育和繁殖中起着重要作用。然而，20E对生殖滞育的影响及其调控机制尚未完全明确。[昆虫名称]是内蒙古草原上的一种新害虫，它以 obligatory reproductive diapause形式夏蛰。在本研究中，从[昆虫名称]中克隆了[基因名称]的完整开放阅读框（ORF）序列。应用20E促进了[基因名称]、[基因名称]（[基因名称]）和[基因名称]（[基因名称]）的表达，而降低了[基因名称]（[基因名称]）的表达和脂质含量，导致雌性成虫滞育进入延迟。通过RNAi沉默[基因名称]或[基因名称]可增加[基因名称]的表达和脂质含量，而降低[基因名称]的表达，诱导生殖滞育。这些结果表明，20E可能通过[昆虫名称]中保守的EcR/HR3级联介导生殖滞育。
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