Ma Hong-Yue, Li Yan-Yan, Li Ling, Tan Yao, Pang Bao-Ping
Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China.
Insects. 2021 Mar 11;12(3):237. doi: 10.3390/insects12030237.
Juvenile hormone (JH) signaling plays an important role in regulation of reproductive diapause in insects. However, we have little understanding of the effect of JH on gene expression at the transcriptome level in diapause. is a new pest in the Inner Mongolia grasslands with obligatory summer diapause in the adult stage. Topical application of a JH analog methoprene at the pre-diapause stage delayed the adults entering diapause and inhibited lipid accumulation whereas it did not during diapause. Using Illumina sequencing technology and bioinformatics tools, 54 and 138 differentially expressed genes (DEGs) were detected at 1 and 2 d after treatment, respectively. The KEGG analysis showed that the DEGs were mainly enriched in the metabolism pathways. qRT-PCR analysis indicated that methoprene promoted the expression of genes encoding vitellogenin, fork head transcription factor and Krüppel homolog 1, whereas suppressed the expression of genes encoding juvenile hormone-binding protein, juvenile hormone esterase, juvenile hormone acid methyltransferase, juvenile hormone epoxide hydrolase and fatty acid synthase 2. These results indicate that JH signaling plays an important role in regulating reproductive diapause of .
保幼激素（JH）信号传导在昆虫生殖滞育的调控中起着重要作用。然而，我们对滞育过程中保幼激素在转录组水平上对基因表达的影响了解甚少。[昆虫名称]是内蒙古草原上的一种新害虫，成虫阶段有 obligatory 夏季滞育。在滞育前阶段局部施用保幼激素类似物烯虫酯可延迟成虫进入滞育并抑制脂质积累，而在滞育期间则没有这种作用。利用Illumina测序技术和生物信息学工具，分别在处理后1天和2天检测到54个和138个差异表达基因（DEG）。KEGG分析表明，差异表达基因主要富集在代谢途径中。qRT-PCR分析表明，烯虫酯促进了编码卵黄原蛋白、叉头转录因子和Krüppel同源物1的基因的表达，而抑制了编码保幼激素结合蛋白、保幼激素酯酶、保幼激素酸甲基转移酶、保幼激素环氧化物水解酶和脂肪酸合酶2的基因的表达。这些结果表明，保幼激素信号传导在调节[昆虫名称]的生殖滞育中起着重要作用。
