González-Inostroz Daniel, Sandoval-García Flavio, Corona-Meraz Fernanda-Isadora, Vázquez Del Mercado Mónica, Guzmán-Muñiz Jorge, Guzmán-Ornelas Milton Omar, Castañeda-Arellano Rolando, Bañuelos-Pineda Jacinto, Peña-Nava Miguel, Martín-Márquez Beatriz-Teresita
Departamento de Biología Molecular y Genómica, Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico.
Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico.
Int J Mol Sci. 2024 Dec 5;25(23):13080. doi: 10.3390/ijms252313080.
The BALB/c model of pristane-induced lupus (PIL) exhibits cognitive impairment features resembling neuropsychiatric lupus (NPLSE). Osteopontin (OPN) is associated with disease activity in SLE; however, its involvement in NPLSE is not yet entirely determined. Our study aims to elucidate the contribution of full-length OPN (OPN-FL) plasma expression, OPN N-half, and to cognitive impairment in the PIL mice model. A total of 76 female BALB/c mice were divided into pristane (P), pristane plus lipopolysaccharide (P plus LPS) and control (C) groups. In behavioral tests, the P group showed cognitive and visuospatial memory impairment. Elevated plasma OPN FL levels were found in P compared to C groups (177.7 ± 90.1 vs. 105.9 ± 56.8 ng/mL, = 0.009) and OPN N-half was different between P and C groups (673.5 ± 144.6 vs. 624.5 ± 377.7 ng/mL, = 0.028) and P plus LPS and C groups (624.5 ± 377.7 vs. 381.4 ± 205.0 ng/mL, = 0.001). Anti-Sm correlated with OPN-FL (r = 0.269, = 0.0150). The relative expression of in the brain was 2.5 and 2.7-fold higher in P and P plus LPS groups, respectively. The evidence suggests that OPN is related to cognitive impairment in PIL mice and might play a relevant role in the detrimental neurological conditions of NPSLE.
pristane诱导的狼疮（PIL）的BALB/c模型表现出类似于神经精神性狼疮（NPLSE）的认知障碍特征。骨桥蛋白（OPN）与系统性红斑狼疮（SLE）的疾病活动相关；然而，其在NPLSE中的作用尚未完全确定。我们的研究旨在阐明全长OPN（OPN-FL）血浆表达、OPN N端以及在PIL小鼠模型中对认知障碍的作用。总共76只雌性BALB/c小鼠被分为pristane（P）组、pristane加脂多糖（P加LPS）组和对照组（C）。在行为测试中，P组表现出认知和视觉空间记忆障碍。与C组相比，P组血浆OPN FL水平升高（177.7±90.1对105.9±56.8 ng/mL，P = 0.009），P组和C组之间的OPN N端存在差异（673.5±144.6对624.5±377.7 ng/mL，P = 0.028），P加LPS组和C组之间也存在差异（624.5±377.7对381.4±205.0 ng/mL，P = 0.001）。抗Sm与OPN-FL相关（r = 0.269，P = 0.0150）。P组和P加LPS组大脑中[具体物质]的相对表达分别高出2.5倍和2.7倍。证据表明，OPN与PIL小鼠的认知障碍有关，可能在NPSLE的有害神经状况中起相关作用。
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