Potyka Katarzyna, Dąbek Józefa
Faculty of Health Sciences in Katowice, Medical University of Silesia in Katowice, 40-635 Katowice, Poland.
Department of Cardiology, Faculty of Health Sciences in Katowice, Medical University of Silesia in Katowice, 40-635 Katowice, Poland.
Int J Mol Sci. 2024 Dec 5;25(23):13102. doi: 10.3390/ijms252313102.
Coronary artery disease and its complications are one of the most common causes of morbidity and death worldwide. The aims of this study were to assess the transcriptional activity of the studied TNF-α genes and their receptors in patients with various degrees of coronary artery disease and in the control group, as well as to attempt to link it with the size of the left ventricular ejection fraction and the number of diseased coronary arteries. Taking into account the inclusion and exclusion criteria, a total of 240 people (100%) qualified for this study. For proper interpretation of the results of the molecular analyzes, the study group (240, 100%) was divided into a control group (C: = 60; 25%), a group of patients with early coronary artery disease (W: = 60; 25%), a group with stable coronary artery disease (S: = 60; 25%), and a group of patients with acute coronary syndrome (ACS: = 60; 25%). The transcriptional activity of the TNF-α genes and their receptors was assessed in peripheral blood mononuclear cells by a quantitative real-time polymerase chain reaction. The expression of the studied genes was inferred from the number of mRNA copies per 1 ug of total RNA. The analysis of the obtained results showed a significant increase in the transcriptional activity of the TNF-α gene with the severity of coronary artery disease, accompanied by a decrease in the activity of its receptor genes. Taking into account the number of affected coronary arteries and the size of the ejection fraction in the examined patients, there were no statistically significant differences in the transcriptional activity of the TNF-α receptor gene type I and II. The observed increase in the transcriptional activity of the TNF-α gene with a concomitant decrease in the activity of its receptor genes with the advancement of coronary artery disease, compared to the control group, may indicate their significant participation in the development and progression of the disease and constitute a useful marker in non-invasive, early diagnostics. In the patients of the study group, no changes in the transcriptional activity of the TNF-α genes and their receptors were demonstrated depending on the number of diseased coronary arteries and the size of the ejection fraction of the left ventricle.
冠状动脉疾病及其并发症是全球发病和死亡的最常见原因之一。本研究的目的是评估不同程度冠状动脉疾病患者和对照组中所研究的肿瘤坏死因子-α（TNF-α）基因及其受体的转录活性，并尝试将其与左心室射血分数大小和病变冠状动脉数量联系起来。考虑到纳入和排除标准，共有240人（100%）符合本研究条件。为了正确解释分子分析结果，研究组（240人，100%）被分为对照组（C：60人；25%）、早期冠状动脉疾病患者组（W：60人；25%）、稳定型冠状动脉疾病患者组（S：60人；25%）和急性冠状动脉综合征患者组（ACS：60人；25%）。通过定量实时聚合酶链反应在外周血单核细胞中评估TNF-α基因及其受体的转录活性。从每1微克总RNA的mRNA拷贝数推断所研究基因的表达。对所得结果的分析表明，TNF-α基因的转录活性随冠状动脉疾病严重程度显著增加，并伴有其受体基因活性降低。考虑到受检患者中病变冠状动脉数量和射血分数大小，I型和II型TNF-α受体基因的转录活性在统计学上无显著差异。与对照组相比，随着冠状动脉疾病进展，观察到TNF-α基因转录活性增加，同时其受体基因活性降低，这可能表明它们在疾病发生和发展中起重要作用，并构成无创早期诊断的有用标志物。在研究组患者中，未发现TNF-α基因及其受体的转录活性因病变冠状动脉数量和左心室射血分数大小而发生变化。
