Tian Qian, Han Wei, Zhou Shuai, Yang Liu, Wang Donghao, Zhou Wen, Wang Zhezhi
Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710062, China.
Int J Mol Sci. 2024 Dec 9;25(23):13223. doi: 10.3390/ijms252313223.
Danshen ( Bunge) is a perennial herbaceous plant of the Salvia genus in the family Lamiaceae. Its dry root is one of the important traditional Chinese herbal medicines with a long officinal history. The yield and quality of are influenced by various factors, among which drought is one of the most significant types of abiotic stress. Based on the transcriptome database of , our research group discovered a carotenoid cleavage dioxygenase gene, , belonging to the carotenoid cleavage oxygenase (CCO) gene family which is highly responsive to drought stress on the basis of our preceding work. Here, we identified 26 CCO genes according to the whole-genome database of . The expression pattern of showed that this gene is strongly overexpressed in the aboveground tissue of . And by constructing overexpression strains, it was shown that the overexpression of not only promotes the synthesis of abscisic acid and increases plant antioxidant activity but also regulates the synthesis of the secondary metabolites tanshinone and phenolic acids in . In summary, this study is the first in-depth and systematic identification and investigation of the CCO gene family in . The results provide useful information for further systematic research on the function of CCO genes and provide a theoretical basis for improving the yield and quality of .
丹参（Bunge）是唇形科鼠尾草属的一种多年生草本植物。其干燥根是一种具有悠久药用历史的重要传统中药材。丹参的产量和质量受多种因素影响，其中干旱是最重要的非生物胁迫类型之一。基于丹参的转录组数据库，我们的研究小组在之前工作的基础上发现了一个类胡萝卜素裂解双加氧酶基因SmCCD1，它属于类胡萝卜素裂解氧化酶（CCO）基因家族，对干旱胁迫高度响应。在此，我们根据丹参的全基因组数据库鉴定出26个CCO基因。SmCCD1的表达模式表明该基因在丹参地上组织中强烈过表达。通过构建SmCCD1过表达菌株，结果表明SmCCD1的过表达不仅促进脱落酸的合成并提高植物抗氧化活性，还调节丹参中次生代谢产物丹参酮和酚酸的合成。综上所述，本研究首次对丹参中的CCO基因家族进行了深入系统的鉴定和研究。研究结果为进一步系统研究CCO基因的功能提供了有用信息，并为提高丹参的产量和质量提供了理论依据。
