Shou Minyu, Lin Qinzhe, Peng Lulu, Wang Zijie, Xu Ying, Qi Jiaochen, Zhao Degang, Shi Min, Kai Guoyin
Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2024 Dec 6;25(23):13138. doi: 10.3390/ijms252313138.
In the process of catalyzing carotenoids into various apocarotenoids and other derivatives, carotenoid cleavage dioxygenases (CCDs) play key roles. However, little information on CCDs has been reported in regard to . In this study, a total of 21 CCD genes were identified in the whole genome of , mainly distributed between five chromosomes. Phylogenetic relationship analysis revealed that 21 SmCCD genes were classified into four subfamilies, including SmCCD4, 7, 8, and NCED; the members of the same subfamily show similar gene structures and tertiary structures. The interspecific collinearity with other plant species, such as and was analyzed. -elements analysis demonstrated that the majority were stress response-, light response-, growth-, and development-related. The expression pattern of the SmCCD genes was expressed in the analyzed tissues. Furthermore, the majority of the SmCCD4 subfamily members varied in their expression levels under the treatment of MeJA, YE, and ABA, indicating the potential function of SmCCD4 in the metabolism process of . In general, this study provides a systematic analysis of SmCCD genes and lays the foundation for uncovering the regulation and function of SmCCD genes in .
在将类胡萝卜素催化转化为各种类胡萝卜素衍生物和其他衍生物的过程中，类胡萝卜素裂解双加氧酶（CCDs）发挥着关键作用。然而，关于CCDs在[具体方面]的报道很少。在本研究中，在[物种名称]的全基因组中总共鉴定出21个CCD基因，主要分布在五条染色体之间。系统发育关系分析表明，21个SmCCD基因被分为四个亚家族，包括SmCCD4、7、8和NCED；同一亚家族的成员显示出相似的基因结构和三级结构。分析了与其他植物物种（如[其他物种名称1]和[其他物种名称2]）的种间共线性。顺式作用元件分析表明，大多数元件与胁迫响应、光响应、生长和发育相关。SmCCD基因的表达模式在分析的组织中有所体现。此外，大多数SmCCD4亚家族成员在茉莉酸甲酯（MeJA）、酵母提取物（YE）和脱落酸（ABA）处理下表达水平有所变化，表明SmCCD4在[具体代谢过程]的代谢过程中具有潜在功能。总体而言，本研究对SmCCD基因进行了系统分析，为揭示SmCCD基因在[物种名称]中的调控和功能奠定了基础。
