Lejeune B, Lamy F, Lecocq R, Deschacht J, Leroy F
J Reprod Fertil. 1985 Jan;73(1):223-8. doi: 10.1530/jrf.0.0730223.
Groups of ovariectomized rats were taken as controls or given hormonal treatment mimicking the successive steps in the sequence of ovarian secretions leading to implantation. Total endometrium or separated epithelium and stroma were incubated in vitro with [35S]methionine. Dissolved proteins were submitted to two-dimensional polyacrylamide gel electrophoresis (pH range 5-7), followed by autoradiography. Priming with oestradiol (2 days) and subsequent treatment with progesterone (3 days) enhanced the synthesis of 12 and 14 polypeptides, respectively, which are specific for each of these treatments. Progesterone also suppressed the production of 10 oestrogen-dependent proteins both in the epithelium and the stroma. When an oestrogen-progesterone-oestrogen treatment was given, synthesis of all but 4 of the progesterone-induced polypeptides in the epithelium was inhibited while 5 of the proteins abolished by progesterone in this tissue compartment reappeared. These results are compatible with a mechanism of implantation acting at the epithelial level by lifting of intrauterine inhibition and stimulation by embryotrophic substances.
将去卵巢大鼠分组作为对照，或给予模拟导致着床的卵巢分泌序列连续步骤的激素治疗。将全层子宫内膜或分离的上皮和基质与[35S]甲硫氨酸进行体外孵育。将溶解的蛋白质进行二维聚丙烯酰胺凝胶电泳（pH范围5 - 7），随后进行放射自显影。用雌二醇预处理（2天）并随后用孕酮处理（3天）分别增强了12种和14种多肽的合成，这些多肽分别是这两种处理所特有的。孕酮还抑制了上皮和基质中10种雌激素依赖性蛋白的产生。当给予雌二醇 - 孕酮 - 雌二醇治疗时，上皮中除4种孕酮诱导的多肽外，其余多肽的合成均受到抑制，而在该组织区室中被孕酮消除的5种蛋白质重新出现。这些结果与一种着床机制相符，该机制通过解除子宫内抑制并由胚胎营养物质刺激，作用于上皮水平。
