Lejeune B, Lecocq R, Lamy F, Leroy F
J Reprod Fertil. 1982 Nov;66(2):519-23. doi: 10.1530/jrf.0.0660519.
Ovariectomized rats were given hormonal treatment mimicking progestational ovarian secretions. At maximal sensitivity a decidual reaction was induced by scratching the endometrium. After incubation of tissue with [35S]methionine, proteins were extracted from decidualizing and control endometrium and submitted to two-dimensional polyacrylamide gel electrophoresis (pH range 5--7), followed by staining or autoradiography. A total of about 800 different peptides could be distinguished on the gels. By 24 h after the decidual stimulus the most prominent changes were the appearance of 4 new peptides (mol. wt 12 000, 25 000, 42 000 and 56 000) and the disappearance of 5 others (mol. wt 31 000, 35 000, 100 000, 110 000 and 140 000). This new pattern remained grossly unchanged up to 72 h after decidual induction.
对去卵巢大鼠进行激素处理，模拟妊娠前期卵巢分泌物。在最大敏感性时，通过刮擦子宫内膜诱导蜕膜反应。用[35S]甲硫氨酸孵育组织后，从蜕膜化和对照子宫内膜中提取蛋白质，并进行二维聚丙烯酰胺凝胶电泳（pH范围5-7），然后进行染色或放射自显影。凝胶上总共可区分出约800种不同的肽。在蜕膜刺激后24小时，最显著的变化是出现了4种新肽（分子量分别为12000、25000、42000和56000），同时5种其他肽（分子量分别为31000、35000、100000、110000和140000）消失。这种新模式在蜕膜诱导后72小时内基本保持不变。
