LRP1B mutation is associated with lymph node metastasis in endometrial carcinoma: A clinical next-generation sequencing study.

BackgroundThis study aims to investigate the mutation status and protein expression of low-density lipoprotein receptor-related protein 1B (LRP1B) in endometrial cancer, and analyze its association with lymph node metastasis (LNM) in endometrial cancer.MethodsTargeted next-generation sequencing (NGS) was conducted on both tumor tissues and paired blood DNA obtained from 94 endometrial cancer patients, followed by comprehensive analysis. Additionally, immunohistochemistry (IHC) was used to explore the correlation between LRP1B protein expression levels, its gene mutation status, and LNM.Results mutation was observed in 19 patients (20.2%). Our results revealed that mutation frequencies were significantly different between endometrial cancer with or without LNM (= 0.038). Multivariate analysis indicated that mutation was a favorable predictor (odds ratio 0.09; 95% confidence interval 0.01-0.95; = 0.045) for LNM in endometrial cancer. Further analysis revealed that combination of mutation with clinical variants (LVSI and histological subtype) yielded a higher area under the curve value of 0.871) and patients harboring mutated-type were less likely to develop LNM. On integrated analysis, the concordance between NGS and LRP1B IHC was 73.3%.ConclusionsThis study utilizes targeted NGS to uncover the relationship between mutation and LNM status, contributing to the development of primary prevention and proactive treatment strategies.