正常人精液中一种精子结合蛋白的鉴定与初步表征

Identification and preliminary characterization of a sperm-binding protein in normal human semen.

作者信息

Abrescia P, Lombardi G, De Rosa M, Quagliozzi L, Guardiola J, Metafora S

出版信息

J Reprod Fertil. 1985 Jan;73(1):71-7. doi: 10.1530/jrf.0.0730071.

DOI:10.1530/jrf.0.0730071

Abstract

In the seminal plasma of normal men a protein, immunologically related to a major protein (RSV-IV) secreted from the rat seminal vesicle epithelium, was detected by competition with RSV-IV in a specific radioimmunoassay using a rabbit anti RSV-IV antiserum. The protein was partly (80%) purified by column chromatography; characterization by gel electrophoresis indicated that the protein is slightly basic and has a molecular weight of 140 000. The protein was present on the surface of human spermatozoa as well as in the ejaculates of azoospermic men and it is believed to be a sperm-binding protein.

摘要

在正常男性的精浆中，通过使用兔抗大鼠精囊上皮分泌的主要蛋白质（RSV-IV）抗血清的特异性放射免疫测定法，与RSV-IV竞争，检测到一种与该蛋白质具有免疫相关性的蛋白质。该蛋白质通过柱色谱法进行了部分（80%）纯化；凝胶电泳表征表明该蛋白质略呈碱性，分子量为140000。该蛋白质存在于人类精子表面以及无精子症男性的精液中，据信它是一种精子结合蛋白。