Kastratovic Nikolina, Milosevic-Djordjevic Olivera, Harrell Carl Randall, Djonov Valentin, Volarevic Vladislav
Centar for Harm Reduction of Biological and Chemical Hazards, Department of Genetics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.
Histol Histopathol. 2025 Jul;40(7):1041-1049. doi: 10.14670/HH-18-854. Epub 2024 Nov 27.
Periodontal ligament-derived mesenchymal stem cells (PDL-MSCs) are promising cells with crucial roles in maintaining and repairing periodontal tissue. However, their regenerative capacity can be influenced by various factors, including cigarette smoke and electronic nicotine delivery system (ENDS) aerosols. Smoking and vaping can impair their regenerative potential, and even though ENDS are perceived as safer tobacco products, there is a lack of evidence to guarantee this assumption.
Changes in the viability and proliferation of PDL-MSCs will be investigated after smoke and aerosol generation and cell exposure. In addition, the effects of smoke and aerosols on the immunomodulatory capacity of PDL-MSCs co-cultured with T lymphocytes will be further determined via the evaluation of cytokine profiles and flow cytometry analysis of T-cell phenotypes.
Combustible cigarettes (CCs) induced more severe impairment in the viability and proliferation of PDL-MSCs compared with ENDS. Also, CCs promoted a proinflammatory immune response that could cause tissue damage to progress. On the other hand, ENDS had the potential to generate an immunosuppressive response that would prevent further cell decay.
The regenerative capacity of PDL-MSCs decreased after treatment with both cigarette smoke and ENDS-aerosols. Even though the results demonstrate less severe effects with ENDS, further research is essential to evaluate their safety and impact on the capacity of PDL-MSCs to prevent and restore oral injuries caused by chronic exposure to aerosols.
牙周膜来源的间充质干细胞（PDL-MSCs）是有前景的细胞，在维持和修复牙周组织中起关键作用。然而，它们的再生能力会受到多种因素影响，包括香烟烟雾和电子尼古丁传送系统（ENDS）气溶胶。吸烟和吸电子烟会损害其再生潜力，尽管ENDS被认为是更安全的烟草产品，但缺乏证据支持这一假设。
在产生烟雾和气溶胶并使细胞暴露后，研究PDL-MSCs活力和增殖的变化。此外，通过评估细胞因子谱和T细胞表型的流式细胞术分析，进一步确定烟雾和气溶胶对与T淋巴细胞共培养的PDL-MSCs免疫调节能力的影响。
与ENDS相比，可燃香烟（CCs）对PDL-MSCs的活力和增殖造成更严重的损害。此外，CCs促进促炎免疫反应，可导致组织损伤进展。另一方面，ENDS有可能产生免疫抑制反应，从而防止细胞进一步衰退。
用香烟烟雾和ENDS气溶胶处理后，PDL-MSCs的再生能力下降。尽管结果表明ENDS的影响较轻，但进一步研究对于评估其安全性以及对PDL-MSCs预防和修复长期暴露于气溶胶引起的口腔损伤能力的影响至关重要。
