Turcz 的化学成分及其体外抗氧化、抗炎和细胞毒性活性。
Chemical Constituents of Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities.
机构信息
Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei 230032, China.
出版信息
Molecules. 2023 Mar 16;28(6):2685. doi: 10.3390/molecules28062685.
Three novel compounds () along with twenty-six known compounds, two known steroids () and twenty-four known phenylpropanoids () were isolated from the whole plant of Turcz. The structures of the three new compounds were elucidated on the basis of ESI-MS, HR-ESIMS, 1D and 2D NMR, IR, UV spectroscopic data. The absolute stereochemistry of compound was determined by the Gauge-Including Atomic Orbitals (GIAO) method. The in vitro antioxidant, anti-inflammatory and cytotoxic activities of the isolated compounds were evaluated by DPPH radical-scavenging assay, LPS-activated RAW 264.7 cells model and CCK-8 kit, respectively. Compound showed high antioxidant activity with an SC value of 16.2 ± 1.6 μM. Compound showed considerable anti-inflammatory activity with an IC value of 28.6 ± 3.0 μM. Compounds and displayed potent cytotoxic activity against human NCI-H292, SiHa, A549, and MKN45 cell lines, with the compound having IC values of 17.4 ± 2.4, 22.2 ± 1.1, 9.7 ± 0.9, 9.5 ±0.7 μM, and the compound having all IC values less than 0.1 μM in vitro.
从 Turcz. 的全株植物中分离得到了三个新化合物 () 以及二十六种已知化合物、两种已知甾体 () 和二十四种已知苯丙素 ()。根据 ESI-MS、HR-ESIMS、1D 和 2D NMR、IR、UV 光谱数据，确定了三个新化合物的结构。通过 Gauge-Including Atomic Orbitals (GIAO) 方法确定了化合物 的绝对立体化学。通过 DPPH 自由基清除试验、LPS 激活 RAW 264.7 细胞模型和 CCK-8 试剂盒分别评估了分离化合物的体外抗氧化、抗炎和细胞毒性活性。化合物 表现出高抗氧化活性，SC 值为 16.2 ± 1.6 μM。化合物 表现出相当的抗炎活性，IC 值为 28.6 ± 3.0 μM。化合物 和 对人 NCI-H292、SiHa、A549 和 MKN45 细胞系表现出强烈的细胞毒性活性，化合物 的 IC 值分别为 17.4 ± 2.4、22.2 ± 1.1、9.7 ± 0.9、9.5 ± 0.7 μM，化合物 的所有 IC 值均小于 0.1 μM 体外。
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