Prasetyo Eric Priyo, Saravanan Pravinna, Kharisna Deaniddo, Wijanarko Christina Immee, Kuntjoro Mefina, Hendrijantini Nike, Tjendronegoro Evelyn
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Oral Biol Craniofac Res. 2025 Jan-Feb;15(1):16-21. doi: 10.1016/j.jobcr.2024.11.009. Epub 2024 Nov 29.
Previous studies revealed diabetes mellitus subjects tend to have persistent apical periodontitis. Regenerative stem cells therapy through endodontic procedure is hoped to be a solution. This study assessed bone regeneration in diabetic rats with apical periodontitis through histopathological analysis of osteoblasts and immunohistochemical analysis of runt-related transcription factor 2 (Runx2) and Osterix.
Diabetes mellitus and apical periodontitis was induced on 20 rats. Apical periodontitis was induced on mandibular right first molars under anesthesia. The teeth were left open for 7 days following access cavity and pulp extirpation, then the rats' teeth were endodontically treated and randomly allocated into 4 groups (5 rats per group). The first and second groups was ended at 30 days (C30) and 60 days (C60) and labelled as control. The third and fourth groups was given umbilical cord mesenchymal stem cells and ended at 30 days (T30) and 60 days (T60). The osteoblasts, Runx2 and Osterix were analyzed. ANOVA and Tukey HSD tests were used for analysis. Differences with p values < 0.05 were considered significant.
The number of osteoblasts in the apical area in control groups (C30 and C60) and treatment groups (T30 and T60) showed a significant increase (p < 0.05). The expressions of Runx2 and Osterix in osteoblasts showed a significant increase among the control (C30 and C60) and treatment groups (T30 and T60) (p < 0.05).
Umbilical cord mesenchymal stem cells improve bone regeneration in diabetic animal model with apical periodontitis, in terms of osteoblasts, Runx2 and Osterix.
先前的研究表明糖尿病患者往往易患持续性根尖周炎。通过牙髓治疗进行再生干细胞疗法有望成为一种解决方案。本研究通过对成骨细胞进行组织病理学分析以及对 runt 相关转录因子 2（Runx2）和 Osterix 进行免疫组织化学分析，评估患有根尖周炎的糖尿病大鼠的骨再生情况。
对 20 只大鼠诱导糖尿病和根尖周炎。在麻醉下于下颌右侧第一磨牙诱导根尖周炎。开髓和牙髓摘除后让牙齿开放 7 天，然后对大鼠牙齿进行牙髓治疗并随机分为 4 组（每组 5 只大鼠）。第一组和第二组分别在 30 天（C30）和 60 天（C60）处死并标记为对照组。第三组和第四组给予脐带间充质干细胞并分别在 30 天（T30）和 60 天（T60）处死。对成骨细胞、Runx2 和 Osterix 进行分析。采用方差分析和 Tukey HSD 检验进行分析。p 值<0.05 的差异被认为具有显著性。
对照组（C30 和 C60）和治疗组（T30 和 T60）根尖区域的成骨细胞数量均显著增加（p<0.05）。成骨细胞中 Runx2 和 Osterix 的表达在对照组（C30 和 C60）和治疗组（T30 和 T60）之间均显著增加（p<0.05）。
就成骨细胞、Runx2 和 Osterix 而言，脐带间充质干细胞可改善患有根尖周炎的糖尿病动物模型的骨再生。
