Yacouba Abdourahamane, Zeidou Alassoum Malika, Marou Soumana Boubacar, Moussa Saley Sahada, Ousmane Abdoulaye, Moussa Harouna, Amatagas Saidou, Alhousseini Daouda, Doutchi Mahamadou, Chaibou Salao, Daou Mamane, Brah Souleymane, Adehossi Eric, Olowo-Okere Ahmed, Mamadou Saidou
Faculté des Sciences de la Santé, Université Abdou Moumouni, Niamey, Niger.
LNR-RAM-Niger, Hôpital National Amirou Boubacar Diallo, Niamey, Niger.
Access Microbiol. 2024 Oct 1;6(10). doi: 10.1099/acmi.0.000708.v4. eCollection 2024.
. Macrolide-induced resistance to clindamycin is a well-described mechanism leading to treatment failure. Herein, we determined the frequency and associated factors of inducible clindamycin resistance in Gram-positive cocci in a tertiary care hospital. . A cross-sectional descriptive study was carried out between January and December 2022. d-tests were performed as recommended by EUCAST 2021 guidelines on 100 non-duplicate clinical isolates of Gram-positive cocci to determine the prevalence of methicillin resistance and inducible clindamycin resistance among the collected isolates. . Of the 100 Gram-positive cocci isolates, 56 (56.0%), 17 (17.0%) and 27 (27.0%) were respectively coagulase-negative staphylococci, and spp. Among spp., Group D Streptococci (15.0%) were the most isolated. Methicillin-resistant (MRSA) represented nine (53.0%) of the isolates. Constitutive (cMLSb) and inducible clindamycin resistance (iMLSb) phenotypes were detected in 36 (36.0%) and 14 (14.0 %) of the isolates, respectively. exhibited 38.4% of cMLSb and 13.7% of iMLSb. The result of multivariate analysis showed that age groups, gender, type of samples, provenance, and bacteria, were not significantly associated with Gram-positive cocci iMLSb phenotype. . The study reported for the first time a high prevalence of inducible resistance of Gram-positive cocci strains to clindamycin in Niger Republic. This suggests the urgent need for the implementation of regular screening of these isolates and the wise use of clindamycin in clinical practice.
大环内酯类药物诱导的对克林霉素耐药是一种已充分描述的导致治疗失败的机制。在此，我们确定了一家三级护理医院中革兰氏阳性球菌诱导型克林霉素耐药的频率及相关因素。
2022年1月至12月进行了一项横断面描述性研究。按照欧盟CAST 2021指南的建议，对100株非重复的革兰氏阳性球菌临床分离株进行了d试验，以确定所收集分离株中甲氧西林耐药和诱导型克林霉素耐药的患病率。
在100株革兰氏阳性球菌分离株中，分别有56株（56.0%）、17株（17.0%）和27株（27.0%）为凝固酶阴性葡萄球菌、肠球菌属和链球菌属。在链球菌属中，D组链球菌（15.0%）是分离最多的。耐甲氧西林金黄色葡萄球菌（MRSA）占金黄色葡萄球菌分离株的9株（53.0%）。分别在36株（36.0%）和14株（14.0%）分离株中检测到组成型（cMLSb）和诱导型克林霉素耐药（iMLSb）表型。金黄色葡萄球菌表现出38.4%的cMLSb和13.7%的iMLSb。多因素分析结果显示，年龄组、性别、样本类型、来源和细菌与革兰氏阳性球菌iMLSb表型无显著相关性。
该研究首次报道了尼日尔共和国革兰氏阳性球菌菌株对克林霉素诱导型耐药的高患病率。这表明迫切需要对这些分离株进行定期筛查，并在临床实践中明智地使用克林霉素。
