Diao Jing, Zhang Yanling, Zhang Xiaoli, Jia Shuyuan, Lei Yajuan, Ma Bowen, Li Xiaodong, Zheng Shuguo, Yuan Chao
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, Beijing, PR China.
Department of Periodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, Beijing, PR China.
Oral Dis. 2025 Jul;31(7):2308-2322. doi: 10.1111/odi.15220. Epub 2024 Dec 17.
This study aimed to analyze the subgingival and salivary microbiome and metabolome in diabetic periodontitis patients with varying glycemic levels.
Forty-two diabetic periodontitis patients were sampled of saliva, gingival crevicular fluid (GCF), and blood, and categorized into three groups based on systemic glycemic status. The microbiome was assessed using full-length 16S rRNA gene sequencing. Gas chromatography-mass spectrometry was performed for metabolome analysis.
The similarity in the structure and function of the flora in saliva and GCF was evident under good blood glucose control. Conversely, inadequate blood glucose control demonstrated a more distinct separation from saliva flora. Both salivary and GCF microorganisms exhibited greater periodontal pathogenicity, with salivary metabolites showing stronger associations with inflammation when Hemoglobin A1c (HbA1c) > 6.5%. Some periodontal pathogens, such as Veillonella atypica, showed significantly positive correlations with proinflammatory metabolites, including lactic acid and putrescine, etc. Salivary microbes demonstrated more sensitive responses than GCF to changes in blood glucose levels among type 2 diabetes mellitus (T2DM) patients.
Under active blood glucose control, it indicates lower periodontal pathogenicity and inflammatory correlation in the oral microecology of T2DM patients. Saliva appears to offer superior diagnostic and monitoring value compared to GCF in the context of systemic disease surveillance.
本研究旨在分析不同血糖水平的糖尿病牙周炎患者的龈下和唾液微生物组及代谢组。
对42例糖尿病牙周炎患者的唾液、龈沟液（GCF）和血液进行采样，并根据全身血糖状况分为三组。使用全长16S rRNA基因测序评估微生物组。采用气相色谱 - 质谱联用技术进行代谢组分析。
在血糖控制良好的情况下，唾液和GCF中菌群的结构和功能相似性明显。相反，血糖控制不佳则表现出与唾液菌群更明显的分离。唾液和GCF中的微生物均表现出更强的牙周致病性，当糖化血红蛋白（HbA1c）>6.5%时，唾液代谢产物与炎症的相关性更强。一些牙周病原体，如非典型韦荣球菌，与促炎代谢产物，包括乳酸和腐胺等，呈显著正相关。在2型糖尿病（T2DM）患者中，唾液微生物对血糖水平变化的反应比GCF更敏感。
在积极控制血糖的情况下，表明T2DM患者口腔微生态中的牙周致病性和炎症相关性较低。在全身性疾病监测方面，与GCF相比，唾液似乎具有更高的诊断和监测价值。
