Bixby R L, O'Brien D J
Appl Environ Microbiol. 1979 Nov;38(5):840-5. doi: 10.1128/aem.38.5.840-845.1979.
The effect of fulvic acid, the major fraction of natural soluble organic matter, on the adsorption of MS2 bacteriophage to soil was investigated in controlled laboratory experiments. Batch experiments together with scanning electron microscopy-energy-dispersive X-ray analysis showed that fulvic acid complexed phage, which prevented its adsorption to soil. Phage strongly adsorbed to soil in the absence of fulvic acid. Phage which was complexed with fulvic acid was not irreversibly inactivated and could become viable under proper conditions, illustrating the importance of assay and elution procedures in the recovery of virus from aqueous solutions.
在可控的实验室实验中,研究了天然可溶性有机物的主要成分黄腐酸对MS2噬菌体吸附到土壤上的影响。批量实验结合扫描电子显微镜-能量色散X射线分析表明,黄腐酸与噬菌体形成复合物,从而阻止其吸附到土壤上。在没有黄腐酸的情况下,噬菌体强烈吸附到土壤上。与黄腐酸形成复合物的噬菌体并没有被不可逆地灭活,并且在适当条件下可以恢复活性,这说明了在从水溶液中回收病毒时测定和洗脱程序的重要性。