Guttman-Bass N, Catalano-Sherman J
Appl Environ Microbiol. 1986 Sep;52(3):556-61. doi: 10.1128/aem.52.3.556-561.1986.
The effects of humic acid on poliovirus type 1 recovery from water by Zeta Plus 60S filters were investigated. The humic acid interfered by preventing virus adsorption to the filters, and the interference increased as a function of the amount of humic acid filtered. Humic acid decreased virus adsorption when filtered before the virus, but did not elute virus which had adsorbed to the filters. The effects on virus recovery were not due to alterations in virus titer or neutralizability. The addition of AlCl3, which improved virus recovery by electronegative filters in the presence of humic acid, did not aid in overall virus recovery by the Zeta Plus filters in the presence or absence of humic acid. However, the salt and humic acid in combination improved virus adsorption and concurrently reduced virus elution efficiency. The addition of NaH2PO4 had no direct effect on virus recovery and did not alter the effect of humic acid. In an attempt to identify the components of humic acid responsible for the interference, humic materials were fractionated by size by using Sephadex gel chromatography and dialysis, and the fractions were tested for interfering activity. Interference was not associated with specific size fractions of the humic materials.
研究了腐殖酸对Zeta Plus 60S过滤器从水中回收1型脊髓灰质炎病毒的影响。腐殖酸通过阻止病毒吸附到过滤器上产生干扰，且干扰程度随过滤的腐殖酸量增加而增大。当在病毒之前过滤时，腐殖酸会降低病毒吸附，但不会洗脱已吸附到过滤器上的病毒。对病毒回收的影响并非由于病毒滴度或中和性的改变。添加AlCl3可在腐殖酸存在时通过带负电的过滤器提高病毒回收率，但在有或没有腐殖酸的情况下，对Zeta Plus过滤器的总体病毒回收并无帮助。然而，盐和腐殖酸共同作用可改善病毒吸附并同时降低病毒洗脱效率。添加NaH2PO4对病毒回收没有直接影响，也不会改变腐殖酸的作用。为了确定腐殖酸中造成干扰的成分，通过使用葡聚糖凝胶色谱法和透析按大小对腐殖物质进行分级分离，并对各馏分的干扰活性进行测试。干扰与腐殖物质的特定大小馏分无关。
