Mousa Ayman M, Allemailem Khaled S
Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia; Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.
Tissue Cell. 2025 Apr;93:102673. doi: 10.1016/j.tice.2024.102673. Epub 2024 Dec 10.
Obesity is a major worldwide health problem affecting one billion people. The purported cardioprotective benefits of hawthorn against cardiovascular diseases (CVDs) are controversial and may be attributed to its antioxidant and anti-inflammatory properties. The current study explored the underlying protective mechanisms of hawthorn berry extract (HBE) against obesity-induced cardiac injury in rats. The control group (G1) was fed a regular rat diet ad libitum. An obesity-induced cardiac injury model was established by feeding a high-fat diet (HFD) to rats of group 2 (G2) and group 3 (G3), while rats of G3 and group 4 (G4) received oral doses of HBE (100 mg/kg) for ten weeks. A light microscope was used to estimate the morphological changes in cardiac tissues. The apoptosis and ROS values of cardiomyocytes were estimated using flow cytometry. Also, the antioxidant enzymes, lipid profile, proinflammatory cytokines, and cardiac enzymes were assessed. Feeding of G2 with HFD significantly increased rats' body weight, cardiac inflammation, apoptosis, and fibrosis compared to G1. As well, significant oxidative stress was observed by reducing GPx1, SOD3, CAT, and HDL-C with a substantial increase of TG, TC, LDL-C, IL-1β, IL-6, TNF-α, cTnI, cTnT, and CK-MB serum levels. On the contrary, supplementation of G3 with HBE significantly protected rats against all mentioned changes compared to G2. The current study confirmed several mechanisms of obesity-induced cardiac injury and the tremendous cardioprotective antioxidant, hypolipidemic, anti-inflammatory, antiapoptotic, and antifibrotic impact of HBE against obesity-induced cardiac injury. Therefore, hawthorn could provide a novel dietary supplement against obesity-induced cardiac injury.
肥胖是一个影响全球10亿人的主要健康问题。山楂对心血管疾病（CVD）的所谓心脏保护益处存在争议，可能归因于其抗氧化和抗炎特性。本研究探讨了山楂浆果提取物（HBE）对肥胖诱导的大鼠心脏损伤的潜在保护机制。对照组（G1）随意喂食常规大鼠饲料。通过给第2组（G2）和第3组（G3）的大鼠喂食高脂饮食（HFD）建立肥胖诱导的心脏损伤模型，而G3组和第4组（G4）的大鼠口服HBE（100mg/kg），持续10周。使用光学显微镜评估心脏组织的形态变化。使用流式细胞术评估心肌细胞的凋亡和ROS值。此外，还评估了抗氧化酶、血脂谱、促炎细胞因子和心脏酶。与G1组相比，给G2组喂食HFD显著增加了大鼠的体重、心脏炎症、凋亡和纤维化。同样，通过降低GPx1、SOD3、CAT和HDL-C，同时大幅增加TG、TC、LDL-C、IL-1β、IL-6、TNF-α、cTnI、cTnT和CK-MB血清水平，观察到显著的氧化应激。相反，与G2组相比，给G3组补充HBE显著保护大鼠免受上述所有变化的影响。本研究证实了肥胖诱导的心脏损伤的几种机制，以及HBE对肥胖诱导的心脏损伤具有巨大的心脏保护抗氧化、降血脂、抗炎、抗凋亡和抗纤维化作用。因此，山楂可以提供一种针对肥胖诱导的心脏损伤的新型膳食补充剂。
