Ren Yi, Li Angze, Miao Xiaojing, Huo Longfei, Qin Haokuan, Jiang Hui, Liu Muqing
School of information science and technology, Fudan University, 2005th Songhu Rd, Shanghai 200438, China.
Academy for Engineering and Technology, Fudan University, 220th Handan Rd, Shanghai 200433, China.
J Photochem Photobiol B. 2025 Jan;262:113080. doi: 10.1016/j.jphotobiol.2024.113080. Epub 2024 Dec 8.
Androgenetic alopecia (AGA) is a prevalent hair loss disorder and influenced by genetic, hormonal, and environmental factors. Minoxidil and finasteride have been widely used for treating AGA. However, the side effects associated with these drugs often lead to poor patient compliance. In contrast, photobiomodulation (PBM), due to its safety and non-invasiveness, holds promising prospects for use. Although the promoting effects of PBM on AGA have been reported, the mechanisms by which PBM affects dermal papilla cells (DPCs) remain largely unknown. Hence, this study explored the impacts of both continuous wave (CW) and pulsed wave (PW) PBM on DPCs, and revealed the underlying actions of light parameters in PBM. Orthogonal experiments were conducted to evaluate the effects of CW PBM on DPCs at varying irradiances and doses, indicating that irradiance was the crucial parameter, as well as cell viability and proliferation were maximized at 8 mW/cm and 8 J/cm. For PW PBM, response surface methodology was employed to determine the influences of duty cycles, frequencies, and doses. The findings highlighted frequency as a primary factor, with the optimum cell viability observed at peak irradiance 10 mW/cm, duty cycle 80 %, 500 Hz, and 8.8 J/cm. Notably, PBM could enhance cell viability, proliferation, and migration in DPCs by activating the Wnt/β-Catenin signaling and suppressing Transforming Growth Factor signaling, particularly when applied in pulsed mode. Overall, this study determined the key light parameters that influence PBM effectiveness, further identified the optimal light conditions, and preliminary revealed into the mechanisms of PBM in DPCs, highlighting that PW PBM may be a competitive therapeutic option for alleviating AGA in the future.
雄激素性脱发（AGA）是一种常见的脱发疾病，受遗传、激素和环境因素影响。米诺地尔和非那雄胺已被广泛用于治疗AGA。然而，这些药物的副作用常常导致患者依从性差。相比之下，光生物调节（PBM）因其安全性和非侵入性而具有广阔的应用前景。尽管已有报道PBM对AGA有促进作用，但其影响毛乳头细胞（DPCs）的机制仍 largely未知。因此，本研究探讨了连续波（CW）和脉冲波（PW）PBM对DPCs的影响，并揭示了PBM中光参数的潜在作用。进行了正交实验以评估不同辐照度和剂量下CW PBM对DPCs的影响，结果表明辐照度是关键参数，在8 mW/cm和8 J/cm时细胞活力和增殖达到最大值。对于PW PBM，采用响应面法确定占空比、频率和剂量的影响。研究结果突出了频率是主要因素，在峰值辐照度10 mW/cm、占空比80%、500 Hz和8.8 J/cm时观察到最佳细胞活力。值得注意的是，PBM可通过激活Wnt/β-连环蛋白信号通路和抑制转化生长因子信号通路来增强DPCs的细胞活力、增殖和迁移，尤其是在脉冲模式下应用时。总体而言，本研究确定了影响PBM有效性的关键光参数，进一步确定了最佳光照条件，并初步揭示了PBM在DPCs中的作用机制，突出表明PW PBM可能是未来缓解AGA的一种有竞争力的治疗选择。
