Cai Wenya, Yan Qingshan, Deng Yuhong, Guo Yong
Department of Public Health, Guangzhou Medical University, Guangzhou 511436, China; Department of Health Care, Guangdong Women and Children Hospital, Guangzhou 511442, China.
Department of Health Care, Guangdong Women and Children Hospital, Guangzhou 511442, China; Department of Basic Medicine and Public Health, Jinan University, Guangzhou 510632, China.
Cytokine. 2025 Feb;186:156835. doi: 10.1016/j.cyto.2024.156835. Epub 2024 Dec 16.
Based on current evidence suggesting that bisphenol A (BPA) may contribute to obesity through the modulation of inflammatory markers, this study aims to investigate the correlation between BPA exposure and cellular inflammatory factors in preschool children.
A total of 155 preschool children aged 4-6 years were included. Urine and blood samples were collected. BPA exposure was detected by liquid chromatography-tandem mass spectrometry through urine samples. The levels of six inflammatory cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ) were determined by flow fluorescence technique. The correlation between urinary BPA exposure and cellular inflammatory factors was analyzed using Spearman's correlation and respectively stratified by gender and BMI.
The detection rate of BPA in urine samples was 100 %. The median urinary BPA concentration was 0.48 μg/L(IQR:0.25-1.02 μg/L), and the creatinine-adjusted BPA concentration was 0.94 μg/g(IQR:0.57-1.66 μg/g). BPA level was negatively correlated with IL-10 (r = -0.172, P < 0.05). After stratification by gender, the negative association between BPA exposure and IL-10 was found in females (r = -0.257, P < 0.05), while no association was found in males. According to BMI stratification, BPA exposure in overweight/obese children was positively correlated with IL-6 (r = 0.354, P < 0.05).
Our study demonstrated that BPA exposure in preschool children was correlated with a decrease in levels of IL-10, and this effect was significantly expressed in girls. In addition, BPA exposure in overweight/obese children was correlated with increased levels of IL-6. However, the mechanism between BPA and inflammatory factors remains to be further explored.
基于目前有证据表明双酚A（BPA）可能通过调节炎症标志物导致肥胖，本研究旨在调查学龄前儿童BPA暴露与细胞炎症因子之间的相关性。
共纳入155名4至6岁的学龄前儿童。收集尿液和血液样本。通过液相色谱-串联质谱法检测尿液样本中的BPA暴露情况。采用流式荧光技术测定六种炎症细胞因子（IL-2、IL-4、IL-6、IL-10、TNF-α和IFN-γ）的水平。使用Spearman相关性分析尿液BPA暴露与细胞炎症因子之间的相关性，并分别按性别和BMI进行分层。
尿液样本中BPA的检出率为100%。尿BPA浓度中位数为0.48μg/L（四分位间距：0.25 - 1.02μg/L），肌酐校正后的BPA浓度为0.94μg/g（四分位间距：0.57 - 1.66μg/g）。BPA水平与IL-10呈负相关（r = -0.172，P < 0.05）。按性别分层后，发现女性BPA暴露与IL-10之间存在负相关（r = -0.257，P < 0.05），而男性未发现相关性。根据BMI分层，超重/肥胖儿童的BPA暴露与IL-6呈正相关（r = 0.354，P < 0.05）。
我们的研究表明，学龄前儿童BPA暴露与IL-10水平降低相关，且这种效应在女孩中显著表现。此外，超重/肥胖儿童的BPA暴露与IL-6水平升高相关。然而，BPA与炎症因子之间的机制仍有待进一步探索。
