拓展抗体发现的领域。
Expanding the landscape of antibody discovery.
作者信息
Johnson Shelbe, DeKosky Brandon J
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA, USA.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; The Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA, USA; The Koch Institute for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
出版信息
Cell Rep Methods. 2024 Dec 16;4(12):100936. doi: 10.1016/j.crmeth.2024.100936.
Library:library screening technologies hold substantial promise for paired antibody:antigen discovery, but challenges have persisted. In this issue of Cell Reports Methods, Wagner et al. introduce a method that combines antibody-ribosome-mRNA complexes, antigen cell surface display, and single-cell RNA sequencing to successfully screen diverse antibody gene libraries against a library of viral receptor proteins.
文库
文库筛选技术在配对抗体-抗原发现方面具有巨大潜力，但挑战依然存在。在本期《细胞报告方法》中，瓦格纳等人介绍了一种方法，该方法将抗体-核糖体-mRNA复合物、抗原细胞表面展示和单细胞RNA测序相结合，以成功地针对病毒受体蛋白文库筛选多种抗体基因文库。
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