Kurogi Yoshitomo, Mizuno Yosuke, Hayashi Ryosuke, Goyins Krystal, Okamoto Naoki, Barton Lacy, Niwa Ryusuke
Graduate School of Science and Technology, University of Tsukuba, Ibaraki 305-8577, Japan.
Degree Programs in Life and Earth Sciences, Graduate School of Science and Technology, University of Tsukuba, Tsukuba, Japan.
Open Biol. 2024 Dec;14(12):240315. doi: 10.1098/rsob.240315. Epub 2024 Dec 18.
Juvenile hormone (JH) is one of the most essential hormones controlling insect metamorphosis and physiology. While it is well known that JH affects many tissues throughout the insect life cycle, the difference in JH responsiveness and the repertoire of JH-inducible genes among different tissues has not been fully investigated. In this study, we monitored JH responsiveness using transgenic flies carrying a () construct. Our data highlight the high responsiveness of the epithelial cells within the seminal vesicle, a component of the male reproductive tract, to JH. Specifically, we observe an elevation in the JHRE-GFP signal within the seminal vesicle epithelium upon JH analogue administration, while suppression occurs upon knockdown of a gene encoding the intracellular JH receptor, . Starting from published transcriptomic and proteomics datasets, we next identified as a JH-response gene expressed in the seminal vesicle epithelium, suggesting insect seminal vesicles undergo metabolic regulation by JH. Together, this study sheds new light on the biology of the insect reproductive regulatory system.
保幼激素（JH）是控制昆虫变态和生理机能的最重要激素之一。虽然众所周知JH在昆虫整个生命周期中会影响许多组织，但不同组织之间JH反应性的差异以及JH诱导基因的种类尚未得到充分研究。在本研究中，我们使用携带（）构建体的转基因果蝇监测JH反应性。我们的数据突出了精囊（雄性生殖道的一个组成部分）内的上皮细胞对JH具有高反应性。具体而言，我们观察到在施用JH类似物后，精囊上皮内的JHRE-GFP信号升高，而在编码细胞内JH受体的基因敲低后则发生信号抑制。从已发表的转录组学和蛋白质组学数据集出发，我们接下来鉴定出作为在精囊上皮中表达的JH反应基因，这表明昆虫精囊受到JH的代谢调节。总之，这项研究为昆虫生殖调节系统的生物学研究提供了新的线索。
