Suppr超能文献

果蝇细胞图谱:成年果蝇的单细胞转录组图谱。

Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly.

机构信息

Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, CA 94305, USA.

Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA.

出版信息

Science. 2022 Mar 4;375(6584):eabk2432. doi: 10.1126/science.abk2432.

Abstract

For more than 100 years, the fruit fly has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula , that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type-related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the community and serves as a reference to study genetic perturbations and disease models at single-cell resolution.

摘要

一百多年来,果蝇一直是研究最多的模式生物之一。在这里,我们展示了成年果蝇 Tabula 的单细胞图谱,该图谱包括来自 15 个单独解剖的性组织以及整个头部和身体的 580,000 个核,注释了 >250 种不同的细胞类型。我们对整个动物的细胞类型相关基因特征和转录因子标记,以及性别二态性进行了深入分析。对组织(如血液和肌肉细胞)之间常见细胞类型的分析揭示了罕见的细胞类型和组织特异性亚型。该图谱为社区提供了有价值的资源,并可作为研究遗传扰动和疾病模型的参考,分辨率达到单细胞水平。

相似文献

1
Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly.
Science. 2022 Mar 4;375(6584):eabk2432. doi: 10.1126/science.abk2432.
7
Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization.
PLoS Genet. 2021 Feb 18;17(2):e1009338. doi: 10.1371/journal.pgen.1009338. eCollection 2021 Feb.
10
Whole-brain annotation and multi-connectome cell typing of Drosophila.
Nature. 2024 Oct;634(8032):139-152. doi: 10.1038/s41586-024-07686-5. Epub 2024 Oct 2.

引用本文的文献

1
Single-cell atlas in the living fossil Yangtze sturgeon provides insight into the evolution of fish.
Genome Biol. 2025 Sep 10;26(1):277. doi: 10.1186/s13059-025-03764-7.
3
A multi-tissue single-cell expression atlas in cattle.
Nat Genet. 2025 Sep 5. doi: 10.1038/s41588-025-02329-5.
5
Cell type specific allometry controls sex-differences in body size.
bioRxiv. 2025 Aug 25:2025.08.25.671808. doi: 10.1101/2025.08.25.671808.
6
Neuropeptide-mediated synaptic plasticity regulates context-dependent mating behaviors in Drosophila.
PLoS Biol. 2025 Sep 4;23(9):e3003330. doi: 10.1371/journal.pbio.3003330. eCollection 2025 Sep.
7
SIFa peptidergic neurons orchestrate the internal states and energy balance of male Drosophila melanogaster.
PLoS Biol. 2025 Sep 4;23(9):e3003345. doi: 10.1371/journal.pbio.3003345. eCollection 2025 Sep.
8
Impacts of mitochondrial dysfunction on axonal microtubule bundles as a potential mechanism of neurodegeneration.
Front Neurosci. 2025 Aug 19;19:1631752. doi: 10.3389/fnins.2025.1631752. eCollection 2025.
10
Establishing single cell RNA transcriptomics: a brief guide.
Front Zool. 2025 Sep 2;22(1):25. doi: 10.1186/s12983-025-00579-x.

本文引用的文献

1
Decoding gene regulation in the fly brain.
Nature. 2022 Jan;601(7894):630-636. doi: 10.1038/s41586-021-04262-z. Epub 2022 Jan 5.
2
A stony coral cell atlas illuminates the molecular and cellular basis of coral symbiosis, calcification, and immunity.
Cell. 2021 May 27;184(11):2973-2987.e18. doi: 10.1016/j.cell.2021.04.005. Epub 2021 May 3.
3
4
FlyBase: updates to the Drosophila melanogaster knowledge base.
Nucleic Acids Res. 2021 Jan 8;49(D1):D899-D907. doi: 10.1093/nar/gkaa1026.
5
A human cell atlas of fetal gene expression.
Science. 2020 Nov 13;370(6518). doi: 10.1126/science.aba7721.
6
A single-cell atlas and lineage analysis of the adult Drosophila ovary.
Nat Commun. 2020 Nov 6;11(1):5628. doi: 10.1038/s41467-020-19361-0.
7
Neuronal diversity and convergence in a visual system developmental atlas.
Nature. 2021 Jan;589(7840):88-95. doi: 10.1038/s41586-020-2879-3. Epub 2020 Nov 4.
8
Transcriptional Programs of Circuit Assembly in the Drosophila Visual System.
Neuron. 2020 Dec 23;108(6):1045-1057.e6. doi: 10.1016/j.neuron.2020.10.006. Epub 2020 Oct 29.
9
Single-cell RNA sequencing in Drosophila: Technologies and applications.
Wiley Interdiscip Rev Dev Biol. 2021 Sep;10(5):e396. doi: 10.1002/wdev.396. Epub 2020 Sep 16.
10
Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila.
Nat Commun. 2020 Sep 8;11(1):4483. doi: 10.1038/s41467-020-18135-y.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验