Echetebu Z O, Moss D W
Clin Chim Acta. 1979 Aug 1;95(3):433-41. doi: 10.1016/0009-8981(79)90193-1.
Up to three zones of gamma-glutamyltransferase activity were present in 89 samples of human serum after agarose-gel electrophoresis at pH 8.6. Their mobilities relative to albumin were zero, 0.3--0.5, and 0.7--0.9. Incubation of human liver tissue in serum increased the activity of the zones with zero and 0.7--0.9 mobilities, and transiently, of the zone of intermediate mobility. More prolonged incubation caused the intermediate zone to decline, and produced new zones of mobility greater than that of albumin which were not seen in native sera. The mobility of partially-purified liver gamma-glutamyltransferase incubated in serum or protein-free solutions was 0.7--0.8. The intermediate zone was not produced when liver tissue was incubated in protein-free solutions, nor with the purified enzyme in serum or protein-free solutions. The possible relevance of these observations to the electrophoretic patterns of gamma-glutamyltransferase in pathological sera is discussed.
在pH 8.6条件下进行琼脂糖凝胶电泳后，89份人血清样本中出现了多达三个γ-谷氨酰转移酶活性区带。它们相对于白蛋白的迁移率分别为零、0.3 - 0.5以及0.7 - 0.9。将人肝组织置于血清中孵育，使迁移率为零和0.7 - 0.9的区带活性增加，中间迁移率区带的活性短暂增加。更长时间的孵育导致中间区带活性下降，并产生了迁移率高于白蛋白的新区带，这些新区带在天然血清中未见。在血清或无蛋白溶液中孵育的部分纯化肝γ-谷氨酰转移酶的迁移率为0.7 - 0.8。当肝组织在无蛋白溶液中孵育时，以及纯化酶在血清或无蛋白溶液中孵育时，均未产生中间区带。讨论了这些观察结果与病理血清中γ-谷氨酰转移酶电泳图谱的可能相关性。
