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微囊化木霉菌种作为优化生物防治应用策略的可行性

Viability of microencapsulated species of Trichoderma as a strategy to optimize use in biological control.

作者信息

Pinotti Thalesram Izidoro, de Oliveira Sousa Tiago, Fonseca Wéverson Lima, Nascimento Thiago Pajeú, Silva Helane França, Brandão Costa Romero Marcos Pedrosa, Santos Alice Maria Gonçalves

机构信息

Campus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, PI, 64900-000, Brazil.

Colégio Técnico de Bom Jesus, Federal University of Piauí, PI, 64900-000, Bom Jesus, Brazil.

出版信息

Braz J Microbiol. 2025 Mar;56(1):11-21. doi: 10.1007/s42770-024-01594-6. Epub 2024 Dec 17.

DOI:10.1007/s42770-024-01594-6
PMID:39690323
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11885768/
Abstract

The increasing use of chemical fungicides without effective control of phytopathogens has led to the development of resistance in microorganisms. As a promising alternative, products formulated with Trichoderma have emerged for their sustainable and effective potential in integrated disease management. However, the predominant formulations do not offer the necessary protection against abiotic factors. In this study, we investigated Trichoderma species encapsulated in sodium alginate through storage viability experiments and their antagonistic potential. The viability and storage conditions of the capsules were evaluated by plating at 5, 15, 30, 45, 60, and 150 days after production, kept in dry or refrigerated environments. The antagonistic potential was determined by the culture pairing method using the phytopathogen Fusarium sp. The results demonstrated that it was possible to maintain the viability of the conidia, with no differences between storage environments. Additionally, the capsule provided UV protection to the conidia encapsulated species possess antagonistic potential, inhibiting 52.54% of Fusarium sp. growth. Consequently, encapsulation is an alternative formulation method that ensures the viability of Trichoderma conidia and optimizes its use in biological control.

摘要

在没有有效控制植物病原体的情况下,化学杀菌剂的使用日益增加,导致微生物产生了抗性。作为一种有前景的替代方法,含有木霉的制剂因其在综合病害管理中的可持续和有效潜力而出现。然而,主要的制剂并不能提供针对非生物因素的必要保护。在本研究中,我们通过储存活力实验研究了包封在海藻酸钠中的木霉菌种及其拮抗潜力。通过在生产后的第5、15、30、45、60和150天进行平板接种,评估胶囊在干燥或冷藏环境中的活力和储存条件。使用植物病原体镰刀菌通过培养配对法测定拮抗潜力。结果表明,可以维持分生孢子的活力,储存环境之间没有差异。此外,胶囊为包封的分生孢子提供了紫外线保护,这些菌种具有拮抗潜力,可抑制52.54%的镰刀菌生长。因此,包封是一种替代制剂方法,可确保木霉分生孢子的活力并优化其在生物防治中的应用。