Fernando Pincha Devage Sameera Madushan, Piao Mei Jing, Herath Herath Mudiyanselage Udari Lakmini, Kang Kyoung Ah, Ha Kwon-Soo, Chae Sungwook, Hyun Jin Won
Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea.
Department of Molecular and Cellular Biochemistry, Kangwon National University School of Medicine, Chuncheon 24341, Republic of Korea.
Biomol Ther (Seoul). 2025 Jan 1;33(1):221-230. doi: 10.4062/biomolther.2024.053. Epub 2024 Dec 18.
Connecting peptide (C-peptide), a byproduct of insulin biosynthesis, has diverse cellular and biological functions. Particulate matter 2.5 (PM) adversely affects human skin, leading to skin thickening, wrinkle formation, skin aging, and inflammation. This study aimed to investigate the protective effects of C-peptide against PM-induced damage to skin cells, focusing on oxidative stress as a key mechanism. C-peptide mitigated NADPH oxidation and intracellular reactive oxygen species (ROS) production induced by PM. It also suppressed PM-induced NADPH oxidase (NOX) activity and alleviated PM-induced NOX1 and NOX4 expression. C-peptide protected against PM-induced DNA damage, lipid peroxidation, and protein carbonylation. Additionally, C-peptide mitigated PM-induced apoptosis by inhibiting intracellular ROS production. In summary, our findings suggest that C-peptide mitigates PM-induced apoptosis in human HaCaT keratinocytes by inhibiting intracellular ROS production and NOX activity.
连接肽（C肽）是胰岛素生物合成的副产物，具有多种细胞和生物学功能。细颗粒物2.5（PM）对人体皮肤产生不利影响，导致皮肤增厚、皱纹形成、皮肤老化和炎症。本研究旨在探讨C肽对PM诱导的皮肤细胞损伤的保护作用，重点关注氧化应激这一关键机制。C肽减轻了PM诱导的NADPH氧化和细胞内活性氧（ROS）生成。它还抑制了PM诱导的NADPH氧化酶（NOX）活性，并减轻了PM诱导的NOX1和NOX4表达。C肽可防止PM诱导的DNA损伤、脂质过氧化和蛋白质羰基化。此外，C肽通过抑制细胞内ROS生成减轻了PM诱导的细胞凋亡。总之，我们的研究结果表明，C肽通过抑制细胞内ROS生成和NOX活性减轻了PM诱导的人HaCaT角质形成细胞凋亡。
