Kim Yun-A, Oh Sehyuk, Park Gyutae, Park Sanghun, Park Yunhwan, Choi Hyunsoo, Kim Minjung, Choi Jungseok
Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea.
Food Functionality Research Division, Korea Food Research Institute, Wanju 55365, Korea.
J Anim Sci Technol. 2024 Nov;66(6):1257-1272. doi: 10.5187/jast.2023.e115. Epub 2024 Nov 30.
The purpose of this study was comparing performances of three breeds of donor satellite cells for cultured meat and selecting the optimal donor and providing insight into the selection of donors for cultured meat production. Cattle muscle satellite cells were isolated from the muscle tissue of Hanwoo, Holstein, and Jeju black cattle, and then sorted by fluorescence activated cell sorting (FACS). Regarding proliferation of satellite cells, all three breeds showed similar trends. The myogenic potential, based on PAX7 and MYOD mRNA expression levels, was similar or significantly higher for Holstein than other breeds. When the area, width, and fusion index of the myotube were calculated through immunofluorescence staining of myosin, it was expressed upward for Holstein in all experiments except myotube area at passage 8. In addition, it was confirmed that Holstein's muscle satellite cells showed an upward expression in the amount of gene and protein expression related to myogenic. In the case of gene expression of MYOG, DES, and MYH4 known to play a key role in differentiation into muscles, it was confirmed that Holstein's muscle satellite cells expressed higher levels. CAV3, IGF1 and TNNT1, which contribute to hypertrophy and differentiation of muscle cells, showed high expression in Holstein. Our results suggest using cells from Holstein cattle can increase the efficiency of cultured meat production, compared to Hanwoo and Jeju breeds, because the cells exhibit superior differentiation behavior which would lead to greater yields during the maturation phase of bioprocessing.
本研究的目的是比较三种用于培养肉的供体卫星细胞的性能，选择最佳供体，并深入了解培养肉生产中供体的选择。从韩牛、荷斯坦牛和济州黑牛的肌肉组织中分离出牛肌肉卫星细胞，然后通过荧光激活细胞分选（FACS）进行分选。关于卫星细胞的增殖，所有三个品种都表现出相似的趋势。基于PAX7和MYOD mRNA表达水平的生肌潜力，荷斯坦牛与其他品种相似或显著更高。当通过肌球蛋白免疫荧光染色计算肌管的面积、宽度和融合指数时，除第8代肌管面积外，在所有实验中荷斯坦牛的这些指标均呈上升趋势。此外，证实荷斯坦牛的肌肉卫星细胞在与生肌相关的基因和蛋白质表达量上呈上升表达。在已知在肌肉分化中起关键作用的MYOG、DES和MYH4的基因表达方面，证实荷斯坦牛的肌肉卫星细胞表达水平更高。有助于肌肉细胞肥大和分化的CAV3、IGF1和TNNT1在荷斯坦牛中高表达。我们的结果表明，与韩牛和济州牛品种相比，使用荷斯坦牛的细胞可以提高培养肉的生产效率，因为这些细胞表现出优异的分化行为，这将在生物加工的成熟阶段带来更高的产量。
