Shao Qiuyu, Dong Chunbo, Han Yanfeng, Zhang Yanwei
Key Laboratory of Development and Utilization of Biological Resources in Colleges and Universities of Guizhou Province/Key Laboratory of Ecology and Management on Forest Fire in Higher Education Institutions of Guizhou Province, Guizhou Education University, Guiyang, China.
Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang, China.
Front Microbiol. 2024 Dec 3;15:1470450. doi: 10.3389/fmicb.2024.1470450. eCollection 2024.
, an important tree, faces serious threat to its growth from environmental stress, particularly climate change. Using plant microbes to enhance host adaptation to respond climate change challenges has been recognized as a viable and sustainable strategy. However, it is still unclear how the perennial tree microbiota varies across phenological stages and the links between respective changes in aboveground and belowground niches. Here, we sequenced 27 root and 27 leaf samples of using 16S rRNA and ITS amplicon sequencing techniques. These samples were obtained from the three main phenological stages of leaves, including leaf growing, aging and decomposing stages. Results showed that the diversity, composition, and function of the leaf microbiota of showed more obvious changes at three phenological time points compared to roots. Regarding alpha diversity, the root microbiota showed no difference across three sampling stages, while the leaf microbiota varied with sampling stages. Regarding beta diversity, the root microbiota clustered from different sampling stages, while the leaf microbiota exhibited distinct separation. Regarding composition and function, the dominant taxa and main functions of the root microbiota were the same in three sampling stages, while the leaf microbiota in the decomposing stage was obviously different from the remaining two stages. Additionally, taxa overlap and source-sink relationship existed between microbiota. Specifically, the degree of overlap among root microbiota was higher than that of leaf microbiota in three sampling stages. The bidirectional source-sink relationship that existed between the root and leaf niches varied with sampling stage. During the leaf growing and aging stages, the proportion of microbial members migrating from roots to leaves was higher than the proportion of members migrating from leaves to roots. During the leaf decomposing stage, the migration characteristics of the fungal community between the root and leaf niches maintained the same as in the remaining two stages, but the proportion of bacterial members migrating from leaves to roots was significantly higher than that of members migrating from roots to leaves. Our findings provide crucial foundational information for utilizing microbiota to benefit their host under climate change challenges.
[具体树种名称]是一种重要的树木，其生长面临来自环境压力，尤其是气候变化的严重威胁。利用植物微生物来增强宿主适应能力以应对气候变化挑战已被视为一种可行且可持续的策略。然而，多年生树木微生物群如何在物候阶段间变化以及地上和地下生态位各自变化之间的联系仍不清楚。在此，我们使用16S rRNA和ITS扩增子测序技术对[具体树种名称]的27个根样本和27个叶样本进行了测序。这些样本取自叶片的三个主要物候阶段，包括叶片生长、衰老和分解阶段。结果表明，与根相比，[具体树种名称]叶微生物群的多样性、组成和功能在三个物候时间点表现出更明显的变化。在α多样性方面，根微生物群在三个采样阶段没有差异，而叶微生物群随采样阶段而变化。在β多样性方面，根微生物群在不同采样阶段聚类，而叶微生物群表现出明显的分离。在组成和功能方面，根微生物群的优势类群和主要功能在三个采样阶段相同，而分解阶段的叶微生物群与其余两个阶段明显不同。此外，[具体树种名称]微生物群之间存在类群重叠和源 - 汇关系。具体而言，在三个采样阶段，根微生物群的重叠程度高于叶微生物群。根和叶生态位之间存在的双向源 - 汇关系随采样阶段而变化。在叶片生长和衰老阶段，从根迁移到叶的微生物成员比例高于从叶迁移到根的成员比例。在叶片分解阶段，根和叶生态位之间真菌群落的迁移特征与其余两个阶段保持相同，但从叶迁移到根的细菌成员比例显著高于从根迁移到叶的成员比例。我们的研究结果为在气候变化挑战下利用[具体树种名称]微生物群使其宿主受益提供了关键的基础信息。
