Saeed Intisar K, Ali Yahia H, Abdellatif Muaz Magzoub, Mustafa Alaa, Abdel-Mageed Ahmad M
Department of Biology, College of Science and Arts, Northern Border University, Arar, Saudi Arabia; and, Virology Department, Central Veterinary Research Laboratory, Khartoum.
Onderstepoort J Vet Res. 2024 Dec 6;91(1):e1-e8. doi: 10.4102/ojvr.v91i1.2193.
To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.47% for BVDV, 11.9% for parainfluenza virus type (PIV-3) and 7.6% for respiratory syncytial virus (RSV). Observed coinfections were bovine viral diarrhoea (BVD) and PIV-3 in 8.1%, BVD and RSV in only two samples (1%). Existence of all three viruses was detected in two samples (1%).Contribution: In this study, infection of the three viruses was confirmed in cattle and existence of its coinfection is documented for the first time.
为了调查牛病毒性腹泻病毒（BVDV）、副流感病毒3型（PIV-3）和呼吸道合胞病毒（RSV）的存在情况及其混合感染情况，从三个不同地区的屠宰场收集了总共420份肺炎肺组织样本。使用抗原检测酶联免疫吸附测定（ELISA）试验对样本进行这三种病毒的检测，阳性结果通过荧光抗体试验和聚合酶链反应进一步确认。检测到的流行率为：BVDV为10.47%，副流感病毒3型（PIV-3）为11.9%，呼吸道合胞病毒（RSV）为7.6%。观察到的混合感染情况为：BVD与PIV-3混合感染占8.1%，BVD与RSV混合感染仅在两份样本中出现（1%）。在两份样本中检测到所有三种病毒均存在（1%）。贡献：在本研究中，首次证实了这三种病毒在牛群中的感染及其混合感染的存在情况。
