Graham D A, McShane J, Mawhinney K A, McLaren I E, Adair B M, Merza M
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast.
J Vet Diagn Invest. 1998 Jan;10(1):43-8. doi: 10.1177/104063879801000108.
A single-dilution quantitative enzyme-linked immunosorbent assay (ELISA) system, based on commercial ELISA kits, for the simultaneous detection of seroconversion to bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), parainfluenza-3 virus (PI3V), and infectious bovine rhinotracheitis virus (IBRV) was evaluated by testing acute and convalescent serum pairs from 564 cattle in 145 outbreaks of respiratory disease. Seroconversion to BVDV, BRSV, PI3V and IBRV was detected in 8.0%, 19.0%, 13.7%, and 7.4%, respectively, of serum pairs tested. Seroconversion was detected in 60.7% of herds and 34.6% of animals tested. Infection with 2 or more viruses was found in 46.6% of these herds and in 27.2% of these animals. The majority of BVDV infections (62%) were associated with other virus infections, suggesting that BVDV may potentiate infection with other agents rather than being a primary pathogen of the respiratory tract. The results were compared with those obtained by virus neutralization and hemagglutination inhibition testing, and the sensitivity, specificity, and overall correlation were calculated. Sensitivities of 92%, 95%, 100%, and 100% were obtained for BVDV, BRSV, PI3V, and IBRV, respectively. The corresponding specificity values were 89%, 92%, 86%, and 91%. The overall correlation for each virus was 90%, 93%, 90%, and 93%, respectively. These results demonstrate that this ELISA system may be used successfully to detect seroconversion in serum pairs, highlight the frequency of multiple viral infections in outbreaks of respiratory disease, and provide further evidence of an immunosuppressive role for BVDV infections.
基于商用酶联免疫吸附测定（ELISA）试剂盒，开发了一种单稀释定量ELISA系统，用于同时检测牛病毒性腹泻病毒（BVDV）、牛呼吸道合胞病毒（BRSV）、副流感3型病毒（PI3V）和传染性牛鼻气管炎病毒（IBRV）的血清转化。通过检测145起呼吸道疾病暴发中564头牛的急性期和恢复期血清对，对该系统进行了评估。在所检测的血清对中，分别有8.0%、19.0%、13.7%和7.4%检测到针对BVDV、BRSV、PI3V和IBRV的血清转化。在60.7%的牛群和34.6%的检测动物中检测到血清转化。在这些牛群的46.6%和这些动物的27.2%中发现感染了两种或更多种病毒。大多数BVDV感染（62%）与其他病毒感染有关，这表明BVDV可能增强其他病原体的感染，而不是呼吸道的主要病原体。将结果与通过病毒中和试验和血凝抑制试验获得的结果进行比较，并计算敏感性、特异性和总体相关性。BVDV、BRSV、PI3V和IBRV的敏感性分别为92%、95%、100%和100%。相应的特异性值分别为89%（BVDV）、92%（BRSV）、86%（PI3V）和91%（IBRV）。每种病毒的总体相关性分别为90%（BVDV）、93%（BRSV）、90%（PI3V）和93%（IBRV）。这些结果表明，该ELISA系统可成功用于检测血清对中的血清转化，突出了呼吸道疾病暴发中多种病毒感染的频率，并为BVDV感染的免疫抑制作用提供了进一步证据。
